Liutskaiuva D, Kamzolkina O V, Starodubtseva L I, Todorov T, Danilenko V N
Antibiot Khimioter. 1991 Mar;36(3):8-11.
Experiments on protoplast formation and regeneration in S. erythraeus, strain BTCC-2 (Saccharopolyspora erythrae) were performed under microscopic control at all the stages. It was shown that the highest protoplast titer was provided by the mycelium grown in one step in the absence of glycine. For characterizing the protoplasts formed by the mycelium grown under different conditions, their regeneration capacity was estimated by microscopic examination of the protoplasts after 15-20-hour growth in microchambers and evaluation of the regeneration efficiency 7-10 hours later. Of interest was the fact of spontaneous development of colonies consisting of the protoplast-like cells (L-cells) in 15-20 hours. Such colonies were formed only by the protoplasts grown from the mycelium incubated in one step in the absence of glycine or in the presence of 0.1 per cent of glycine. Such conditions provided also the maximum efficiency of the protoplast regeneration. The long-term storage of protoplasts led to a decrease in their viability.
在显微镜控制下，对红色糖多孢菌（Saccharopolyspora erythrae）BTCC - 2菌株进行了原生质体形成和再生的各个阶段实验。结果表明，在无甘氨酸条件下一步培养的菌丝体可产生最高的原生质体滴度。为了表征在不同条件下生长的菌丝体形成的原生质体，通过在微室中培养15 - 20小时后对原生质体进行显微镜检查，并在7 - 10小时后评估再生效率，来估计它们的再生能力。有趣的是，在15 - 20小时内，由原生质体样细胞（L细胞）组成的菌落会自发形成。这种菌落仅由在无甘氨酸或存在0.1%甘氨酸条件下一步培养的菌丝体生长出的原生质体形成。这些条件也提供了原生质体再生的最大效率。原生质体的长期储存会导致其活力下降。
