Sasaki S, Takagi T, Suzuki M
Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, Japan.
Arch Oral Biol. 1991;36(3):227-31. doi: 10.1016/0003-9969(91)90090-h.
Developing enamel was stained with pH indicators and a banded colour pattern exhibiting alternate acidic (5.8-6.0) and neutral (7.0-7.2) staining was clearly visualized. The pH values of the enamel samples scraped from respective bands were confirmed by measuring them in suspensions with a pH meter. Neutral bands corresponded to red stripes of glyoxal bis(2-hydroxyanil) staining and acidic bands to unstained ones, suggested a correlation of the acidic and neutral stripes with the zones of ruffle-ended and smooth-ended ameloblasts.
正在发育的牙釉质用pH指示剂染色,清晰可见呈现交替酸性(5.8 - 6.0)和中性(7.0 - 7.2)染色的带状颜色模式。从各个带刮下的牙釉质样本的pH值通过用pH计在悬浮液中测量来确认。中性带对应于乙二醛双(2 - 羟基苯胺)染色的红色条纹,酸性带对应于未染色的条纹,这表明酸性和中性条纹与褶边末端和成釉细胞平滑末端的区域相关。