Takano Y, Matsuo S, Wakisaka S, Ichikawa H, Nishikawa S, Akai M
Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Arch Oral Biol. 1988;33(4):231-6. doi: 10.1016/0003-9969(88)90183-5.
Staining patterns of the surface and interior of maturing enamel of these tooth germs were examined using the glyoxal bis(2-hydroxyanil) (GBHA) solution which chelates with calcium loosely bound to hydroxyapatite to form insoluble red precipitates. An intense red, band-like pattern of GBHA staining, 1-2 mm wide, first appeared on the incisal portion of lingual enamel surface as complete loops, concentrically arranged; these gradually increased in number. Most of the later-formed bands encircled the entire periphery of the maturing enamel surface. GBHA also revealed reactive areas on the labio-lingual, cut and ground surface of maturing enamel, corresponding exactly to the surface GBHA bands. GBHA did not stain EDTA-etched surface enamel, but did reveal regular staining patterns on the ground surface, disclosed after EDTA treatment. These observations suggest an intimate correlation between the properties of interior and surface enamel, at least with regard to the state of local calcium. The maturation of bovine incisor enamel may start at the lingual aspect of the tooth crown.
使用乙二醛双（2-羟基苯胺）（GBHA）溶液检查这些牙胚成熟釉质的表面和内部的染色模式，该溶液与松散结合在羟基磷灰石上的钙螯合，形成不溶性红色沉淀。GBHA染色呈现出强烈的红色带状模式，宽1-2毫米，首先出现在舌侧釉质表面的切缘部分，呈完整的环状，同心排列；这些环的数量逐渐增加。大多数后来形成的带环绕着成熟釉质表面的整个周边。GBHA还揭示了成熟釉质的唇舌面、切割面和磨面上的反应区域，与表面GBHA带完全对应。GBHA不会对EDTA蚀刻的表面釉质染色，但在EDTA处理后，确实在磨面上揭示了规则的染色模式。这些观察结果表明，至少在局部钙的状态方面，内部釉质和表面釉质的性质之间存在密切关联。牛切牙釉质的成熟可能从牙冠的舌侧开始。
