Sui W, Boyd C, Wright J T
Department of Pediatric Dentistry, Brauer Hall CB 7450, School of Dentistry, University of North Carolina, Chapel Hill 27599-7450, USA.
J Dent Res. 2003 May;82(5):388-92. doi: 10.1177/154405910308200512.
Regulation of pH is necessary to the production of an environment conducive to enamel growth and mineralization. We hypothesize that abnormal extracellular pH in the enamel matrix of mice with the cystic fibrosis gene knocked out (CF mice) results in altered enamel mineralization. The enamel matrix pH during amelogenesis was studied in 10 normal and 10 CF mice. Freshly dissected incisors were immersed in pH indicator or glyoxal bis (2-hydro-xyanil) (GBHA). The normal mouse enamel matrix pH was generally higher and modulated differently than did the CF mouse enamel. GBHA staining showed that normal mice had 2 well-demarcated bands in the maturation zone that correlated to the neutral pH zones, while CF mice showed no staining. These results indicate that CFTR plays a role in pH regulation during enamel development and that a reduced pH results in a lack of calcium influx during enamel maturation and hypomineralization of the CF incisor enamel.
调节pH值对于营造有利于牙釉质生长和矿化的环境至关重要。我们推测，敲除囊性纤维化基因的小鼠（CF小鼠）牙釉质基质中细胞外pH值异常会导致牙釉质矿化改变。对10只正常小鼠和10只CF小鼠在釉质形成过程中的牙釉质基质pH值进行了研究。将刚解剖的门牙浸入pH指示剂或乙二醛双（2-羟基苯胺）（GBHA）中。正常小鼠牙釉质基质pH值通常较高，且与CF小鼠牙釉质的调节方式不同。GBHA染色显示，正常小鼠在成熟区有两条界限分明的带，与中性pH区相关，而CF小鼠则无染色。这些结果表明，CFTR在牙釉质发育过程中的pH调节中起作用，pH值降低会导致牙釉质成熟过程中钙内流缺乏以及CF门牙牙釉质矿化不足。
