Cockerell G L, Hoover E A, Krakowka S, Olsen R G, Yohn D S
J Natl Cancer Inst. 1976 Nov;57(5):1095-9. doi: 10.1093/jnci/57.5.1095.
Mitogen-induced blast transformation of peripheral blood lymphocytes and quantitative changes in circulating T- and B-cells were studied serially in cats inoculated with feline leukemia virus (FeLV). Concanavalin A-induced blast transformation sharply declined beginning at 5 weeks post inoculation (Pl) in FeLV-infected cats when compared to age-matched uninfected control cats. Similar but less consistent changes were seen in responses to pokeweed mitogen-induced stimulation. In most infected kittens this defect persisted until they died from thymic lymphosarcoma, 15-24 weeks Pl. An early lymphopenia, due primarily to a decrease in circulating B-cells, occurred in infected cats 5-8 weeks Pl. Following a return of total and B-lymphocytes to control values, infected cats developed increased numbers of T-cells at 16 or more weeks Pl, which correlated with circulating lymphoblastic lymphocytes bearing T-cell markers. These results correlated neoplasia arising in a thymus-derived lymphocyte population with mitogenic hyporeactivity in the preneoplastic period and suggested that FeLV-induced immune alterations may be a necessary antecedent of leukemogenesis in the cat.
对接种猫白血病病毒（FeLV）的猫，连续研究了丝裂原诱导的外周血淋巴细胞的母细胞转化以及循环T细胞和B细胞的定量变化。与年龄匹配的未感染对照猫相比，接种FeLV的猫在接种后5周（PI）开始，伴刀豆球蛋白A诱导的母细胞转化急剧下降。在对商陆丝裂原诱导刺激的反应中观察到类似但不太一致的变化。在大多数受感染的小猫中，这种缺陷一直持续到它们在接种后15 - 24周死于胸腺淋巴肉瘤。接种后5 - 8周，受感染的猫出现早期淋巴细胞减少，主要是由于循环B细胞减少。在总淋巴细胞和B淋巴细胞恢复到对照值后，受感染的猫在接种后16周或更长时间T细胞数量增加，这与带有T细胞标志物的循环淋巴母细胞相关。这些结果将源自胸腺的淋巴细胞群体中发生的肿瘤形成与肿瘤前期的有丝分裂反应低下相关联，并表明FeLV诱导的免疫改变可能是猫白血病发生的必要前提。
