Yue G H, Wang G L, Zhu B Q, Wang C M, Zhu Z Y, Lo L C
Molecular population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
Int J Biol Sci. 2008 Aug 28;4(5):279-82. doi: 10.7150/ijbs.4.279.
Self-cloning is quite rare in shrimp, lobsters, crayfish and crabs. Here we report the discovery of four natural clones of red swamp crayfish (Procambarus clarkii), each containing 2-6 genetically identical individuals, during the genotyping of 120 individuals with five microsatellites. The four clones were heterozygote at most of the five microsatellite loci. Phylogenetic analysis using microsatellite genotypes suggests recent origin of the four clones. Sequencing a part of the mitochondrial gene Cox I confirmed that the four clones were from the species Procambarus clarkii.
自我克隆现象在虾、龙虾、小龙虾和螃蟹中相当罕见。在此，我们报告在对120个个体进行五个微卫星基因分型的过程中，发现了四个克氏原螯虾（Procambarus clarkii）的天然克隆体，每个克隆体包含2 - 6个基因相同的个体。这四个克隆体在五个微卫星位点中的大多数位点上都是杂合子。利用微卫星基因型进行的系统发育分析表明这四个克隆体起源较近。对线粒体基因Cox I的部分序列进行测序证实这四个克隆体均来自克氏原螯虾物种。
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