Dai Tian-Hao, Sserwadda Ali, Song Kun, Zang Ya-Nan, Shen Huai-Shun
Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Int J Mol Sci. 2016 Oct 18;17(10):1739. doi: 10.3390/ijms17101739.
Ecdysone receptor and retinoid X receptor are key regulators in molting. Here, full length ecdysone receptor () and retinoid X receptor () cDNAs from were cloned. Full length cDNA of has 2500 bp, encoding 576 amino acid proteins, and full length cDNA of has 2593 bp, in which a 15 bp and a 204 bp insert/deletion splice variant regions in DNA binding domain and hinge domain were identified. The two splice variant regions in result four isoforms: -4, encoding 525, 520, 457 and 452 amino acids respectively. was highly expressed in the hepatopancreas and eyestalk and was highly expressed in the eyestalk among eight examined tissues. Both and had induced expression after eyestalk ablation (ESA) in the three examined tissues. In muscle, and were upregulated after ESA, reached the highest level on day 3 after ESA and increased 33.5-fold relative to day 0, and reached highest the level on day 1 after ESA and increased 2.7-fold relative to day 0. In the hepatopancreas, and deased continuously after ESA, and the expression levels of and were only 0.7% and 1.7% on day 7 after ESA relative to day 0, respectively. In the ovaries, was upregulated after ESA, reached the highest level on day 3 after ESA, increased 3.0-fold relative to day 0, and the expression level of changed insignificantly after ESA ( > 0.05). The different responses of and after ESA indicates that different tissues play different roles (and coordinates their functions) in molting.
蜕皮激素受体和维甲酸X受体是蜕皮过程中的关键调节因子。在此，克隆了来自[具体物种]的全长蜕皮激素受体（）和维甲酸X受体（）cDNA。[具体物种]的全长cDNA有2500 bp，编码576个氨基酸的蛋白质，而[具体物种]的全长cDNA有2593 bp，其中在DNA结合结构域和铰链结构域中鉴定出15 bp和204 bp的插入/缺失剪接变体区域。[具体物种]中的两个剪接变体区域产生四种异构体：-4，分别编码525、520、457和452个氨基酸。在八个检测组织中，[具体物种]在肝胰腺和眼柄中高表达，而[具体物种]在眼柄中高表达。在三个检测组织中，眼柄切除（ESA）后[具体物种]和[具体物种]均有诱导表达。在肌肉中，ESA后[具体物种]和[具体物种]上调，[具体物种]在ESA后第3天达到最高水平，相对于第0天增加了33.5倍，[具体物种]在ESA后第1天达到最高水平，相对于第0天增加了2.7倍。在肝胰腺中，ESA后[具体物种]和[具体物种]持续下降，ESA后第7天[具体物种]和[具体物种]的表达水平相对于第0天分别仅为0.7%和1.7%。在卵巢中，ESA后[具体物种]上调，在ESA后第3天达到最高水平，相对于第0天增加了3.0倍，ESA后[具体物种]的表达水平变化不显著（>0.05）。ESA后[具体物种]和[具体物种]的不同反应表明不同组织在蜕皮过程中发挥不同作用（并协调其功能）。
