Zhong Yizhi, Tang Zhangsheng, Huang Liming, Wang Dapeng, Lu Zhuanling
Guangxi Academy of Fishery Sciences/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, China.
Guangxi Nanning Yanleshang Biotechnology Co. Ltd, Nanning, China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2020 Mar;31(2):48-56. doi: 10.1080/24701394.2020.1721484. Epub 2020 Feb 3.
The red swamp crayfish () is a famous invasive species. However, it has become one of the most important freshwater aquaculture resources in China. Herein, we focus on five artificial cultured populations of in Guangxi, southern China to investigate the genetic diversity based on mitochondrial DNA and microsatellites. The results revealed that the genetic diversity of populations in southern Guangxi (NN and DT) was lower than in central (LZ and LB) and northern (RS) Guangxi. A total of 17 haplotypes were captured from 100 individuals and haplotype 2 mainly existed, and the number of haplotype in NN and DT was less than other populations. The AMOVA showed that genetic variation was determined by within populations. The observed mismatch distribution of overall populations fit the expected distributions, indicating that no obvious demographic expansion, but unimodal was observed in RS, LZ and LB. st among most of groups exhibited moderate differentiation. Nevertheless, the gene flow demonstrated there had extensive gene exchanges between different populations, particularly between LZ and LB. UPGMA tree revealed that NN and DT belonged to the same clade, whereas the remaining populations were in the other clade. According to the genetic diversity, there is a need to improve the germplasm resources of in southern Guangxi.
克氏原螯虾是一种著名的入侵物种。然而，它已成为中国最重要的淡水养殖资源之一。在此，我们聚焦于中国南方广西的五个克氏原螯虾人工养殖群体，基于线粒体DNA和微卫星来研究其遗传多样性。结果显示，广西南部（南宁和德保）的克氏原螯虾群体的遗传多样性低于广西中部（柳州和来宾）和北部（融水）。从100个个体中共捕获到17个单倍型，且主要存在单倍型2，南宁和德保的单倍型数量少于其他群体。分子方差分析表明，遗传变异由群体内部决定。总体群体观察到的错配分布符合预期分布，表明没有明显的种群扩张，但在融水、柳州和来宾观察到单峰分布。大多数群体间的Fst表现出中等程度的分化。然而，基因流表明不同群体之间存在广泛的基因交流，特别是柳州和来宾之间。非加权组平均法树状图显示，南宁和德保属于同一分支，而其余群体在另一个分支。根据遗传多样性，有必要改善广西南部克氏原螯虾的种质资源。
