Adams Nicole E, Homola Jared J, Sard Nicholas M, Nathan Lucas R, Roth Brian M, Robinson John D, Scribner Kim T
Department of Fisheries and Wildlife Michigan State University East Lansing Michigan USA.
U.S. Geological Survey, Wisconsin Cooperative Fishery Research Unit, College of Natural Resources University of Wisconsin-Stevens Point Stevens Point Wisconsin USA.
Evol Appl. 2024 Sep 15;17(9):e70007. doi: 10.1111/eva.70007. eCollection 2024 Sep.
The establishment and spread of invasive species are directly related to intersexual interactions as dispersal and reproductive success are related to distribution, effective population size, and population growth. Accordingly, populations established by r-selected species are particularly difficult to suppress or eradicate. One such species, the red swamp crayfish () is established globally at considerable ecological and financial costs to natural and human communities. Here, we develop a single nucleotide polymorphism (SNP) loci panel for using restriction-associated DNA-sequencing data. We use the SNP panel to successfully genotype 1800 individuals at 930 SNPs in southeastern Michigan, USA. Genotypic data were used to reconstruct pedigrees, which enabled the characterization of mating system and statistical tests for associations among environmental, demographic, and phenotypic predictors and adult reproductive success estimates. We identified juvenile cohorts using genotype-based pedigrees, body size, and sampling timing, which elucidated the breeding phenology of multiple introduced populations. We report a high prevalence of multiple paternity in each surveyed waterbody, indicating polyandry in this species. We highlight the use of newly developed rapid genomic assessment tools for monitoring population reproductive responses, effective population sizes, and dispersal during ongoing control efforts.
入侵物种的建立和扩散与两性间相互作用直接相关，因为扩散和繁殖成功与分布、有效种群大小及种群增长有关。因此，由r选择物种建立的种群特别难以抑制或根除。一种这样的物种，即克氏原螯虾（Procambarus clarkii），已在全球范围内定殖，给自然和人类群落带来了巨大的生态和经济代价。在此，我们利用限制性内切酶相关DNA测序数据为克氏原螯虾开发了一个单核苷酸多态性（SNP）位点面板。我们使用该SNP面板成功地对美国密歇根州东南部1,800个个体的930个SNP进行了基因分型。基因型数据用于重建谱系，这使得能够表征交配系统，并对环境、人口统计学和表型预测因子与成体繁殖成功估计值之间的关联进行统计检验。我们利用基于基因型的谱系、体型和采样时间确定了幼体群组，这阐明了多个引入种群的繁殖物候。我们报告在每个被调查水体中多父现象的发生率很高，表明该物种存在一妻多夫制。我们强调使用新开发的快速基因组评估工具来监测正在进行的控制工作期间的种群繁殖反应、有效种群大小和扩散情况。
