从患有尿路感染和子宫蓄脓病例的犬只以及健康犬只粪便中分离出的大肠杆菌菌株中的毒力因子。
Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs.
作者信息
Siqueira Amanda K, Ribeiro Marcio G, Leite Domingos da S, Tiba Monique R, Moura Claudia de, Lopes Maria Denise, Prestes Nereu Carlos, Salerno Tatiana, Silva Aristeu V da
机构信息
Departamento de Higiene Veterinária e Saúde Pública - FMVZ/UNESP, Caixa Postal 560, CEP: 18.618-000 Botucatu, São Paulo, Brazil.
出版信息
Res Vet Sci. 2009 Apr;86(2):206-10. doi: 10.1016/j.rvsc.2008.07.018. Epub 2008 Sep 9.
The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.
本研究旨在通过聚合酶链反应（PCR）比较从51例犬尿路感染临床病例、52例子宫蓄脓病例以及55份健康犬粪便样本中分离出的158株大肠杆菌中毒力基因的流行情况。在从尿路感染病例中分离出的12株（23.5%）菌株、从子宫蓄脓病例中分离出的19株（36.5%）菌株以及从粪便中分离出的10株（18.）中发现了papC基因。在从子宫蓄脓病例中分离出的3株（5.8%）菌株中观察到了papGII基因，在从尿路感染病例中分离出的10株（19.6%）菌株、从子宫蓄脓病例中分离出的15株（28.8%）菌株以及从粪便中分离出的9株（16.4%）菌株中观察到了papGIII基因。在从尿路感染病例中分离出的22株（43.1%）菌株、从子宫蓄脓病例中分离出的24株（46.1%）菌株以及从粪便中分离出的19株（34.5%）菌株中检测到了sfaS基因。在从尿路感染病例中分离出的17株（33.3%）菌株、从子宫蓄脓病例中分离出的18株（34.6%）菌株以及从粪便中分离出的7株（12.7%）菌株中观察到了hlyA基因，而在从尿路感染病例中分离出的11株（21.6%）菌株、从子宫蓄脓病例中分离出的21株（40.4%）菌株以及从粪便中分离出的9株（16.4%）菌株中检测到了cnf - 1基因。在从尿路感染病例中分离出的12株（23.5%）菌株、从子宫蓄脓病例中分离出的9株（17.3%）菌株以及从粪便中分离出的1株（1.8%）菌株中观察到了iucD基因。在从尿路感染病例中分离出的17株（33.3%）菌株以及从子宫蓄脓病例中分离出的36株（69.9%）菌株中发现了usp基因。
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