犬子宫蓄脓分离的大肠杆菌中致病基因频率及脉冲场凝胶电泳指纹图谱
Frequencies of virulence genes and pulse field gel electrophoresis fingerprints in Escherichia coli isolates from canine pyometra.
作者信息
Maluta Renato P, Borges Clarissa A, Beraldo Lívia G, Cardozo Marita V, Voorwald Fabiana A, Santana André M, Rigobelo Everlon C, Toniollo Gilson H, Avila Fernando A
机构信息
Programa de Pós Graduação em Microbiologia Agropecuária, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
Programa de Pós Graduação em Microbiologia Agropecuária, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
出版信息
Vet J. 2014 Nov;202(2):393-5. doi: 10.1016/j.tvjl.2014.08.016. Epub 2014 Aug 19.
Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones.
大肠杆菌是从犬子宫蓄脓病例中分离出的最常见细菌病原体。对来自巴西犬子宫蓄脓病例的23株大肠杆菌分离株，测定了24个毒力基因的频率和脉冲场凝胶电泳（PFGE）图谱。毒力基因的频率分别为：fimH 91.3%、irp-2 91.3%、fyuA 82.6%、iroN 56.5%、traT 47.8%、usp 39.1%、sfaD/E 34.8%、tsh 34.8%、papC 30.4%、hlyA 30.4%、papGIII 26.1%、cnf-1 26.1%、papE/F 21.7%、iss 21.7%、iutA 17.4%、ompT 17.4%、cvaC 17.4%、hlyF 17.4%、iucD 17.4%、iucC 13.0%、astA 13.0%、papGII 4.3%、afaB/C 0%、papGI 0%。耶尔森菌素（fyuA和irp2）和沙门菌素（iroN）基因的高频率表明，铁摄取系统可能在犬子宫蓄脓的发病机制中起重要作用。19株分离株的PFGE图谱具有异质性，证实来自犬子宫蓄脓的大肠杆菌分离株不太可能是流行克隆。
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