Goshima J, Goldberg V M, Caplan A I
Department of Biology, University Hospitals, Case Western Reserve University, School of Medicine, Cleveland, OH.
Biomaterials. 1991 Mar;12(2):253-8. doi: 10.1016/0142-9612(91)90209-s.
It has been established that, when whole marrow is introduced into porous calcium phosphate ceramic, bone forms on the walls of the pores. To extend earlier studies, bone marrow cells derived from the femora of inbred rats were introduced into tissue culture and the adherent cells cultivated, mitotically expanded, passaged, harvested, placed in small cubes of porous calcium phosphate ceramics and grafted into subcutaneous sites of syngeneic rats. Marrow-derived, cultured mesenchymal cells introduced into ceramics showed strong osteogenic potential, with bone forming in the pore regions of ceramics as early as 2 wk after implantation. Osteogenesis could be observed after the eighteenth passage. With increasing passage number, the initiation of osteogenesis and the apparent rate of bone formation declined and the course of osteogenesis was delayed. In the future, it may be possible to culture marrow cells as a source for reparative cells for implantation back into autologous in vivo sites.
已经确定，当将全骨髓引入多孔磷酸钙陶瓷中时，骨会在孔壁上形成。为了扩展早期研究，将来自近交系大鼠股骨的骨髓细胞引入组织培养，培养贴壁细胞，进行有丝分裂扩增、传代、收获，置于多孔磷酸钙陶瓷小方块中，并移植到同基因大鼠的皮下部位。引入陶瓷的骨髓来源的培养间充质细胞显示出很强的成骨潜力，植入后早在2周就在陶瓷的孔区域形成骨。在第18代后可观察到成骨。随着传代次数的增加，成骨的起始和明显的骨形成速率下降，成骨过程延迟。未来，有可能将骨髓细胞培养为修复性细胞来源，以便植入回自体体内部位。
