Ohgushi H, Okumura M, Tamai S, Shors E C, Caplan A I
Department of Orthopedics, Nara Medical University, Japan.
J Biomed Mater Res. 1990 Dec;24(12):1563-70. doi: 10.1002/jbm.820241202.
To investigate the bone formation ability of porous hydroxyapatite (HA) and tricalcium phosphate (TCP), ceramic discs were implanted with or without rat marrow cells into subcutaneous sites in syngeneic rats. The discs of HA and TCP had identical microstructures: pore size was 190-230 microns, porosity was 50-60%, and they were fully interconnected. Implants without marrow cells (discs themselves) did not show bone formation, whereas implants with marrow cells showed bone formation in the pores of the ceramics. The bone formation of both HA and TCP occurred initially on the surface of the ceramic and progressed towards the center of the pore. The de novo bone was quantitated from decalcified serial sections of the implants. One month after implantation with marrow cells, the percentage fractions of the pore area filled with bone for implanted HA and TCP were 16.9 and 15.1, respectively. At 2 months after implantation with marrow cells, the fractions of bone were 34.3 and 30.9, respectively. These results indicate that both HA and TCP ceramics can show comparable osteogenic ability in the presence of marrow cells.
为研究多孔羟基磷灰石（HA）和磷酸三钙（TCP）的骨形成能力，将植入或未植入大鼠骨髓细胞的陶瓷盘皮下植入同基因大鼠体内。HA和TCP盘具有相同的微观结构：孔径为190 - 230微米，孔隙率为50 - 60%，且它们完全相互连通。未植入骨髓细胞的植入物（盘本身）未显示骨形成，而植入骨髓细胞的植入物在陶瓷孔隙中显示出骨形成。HA和TCP的骨形成最初都发生在陶瓷表面，并向孔隙中心发展。从植入物的脱钙连续切片中对新生骨进行定量分析。植入骨髓细胞1个月后，植入HA和TCP的孔隙区域被骨填充的百分比分别为16.9和15.1。植入骨髓细胞2个月后，骨的比例分别为34.3和30.9。这些结果表明，在存在骨髓细胞的情况下，HA和TCP陶瓷都能表现出相当的成骨能力。
