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[在环己酰亚胺抑制翻译作用下，染色体外环状DNA中高度重复序列数量的增加]

[Increase in the quantity of highly repetitive sequences in extrachromosomal circular DNAs under inhibition of translation by cycloheximide].

作者信息

Krokhina T B, Raevskaia G B, Chernyĭ D I, Gar'kavtsev I V

出版信息

Biull Eksp Biol Med. 1991 May;111(5):530-2.

PMID:1878571
Abstract

Small polydispersed circular DNA(spcDNA) was isolated from cultivated HeLa cells. Cells were treated by cycloheximide in concentrations of 1 and 50 micrograms/ml. Gradient fractions were dot blotted to nitrocellulose filters and were hybridized with different repetitive DNAs. The pool of repetitive DNA sequences in fraction of spcDNA increased for cycloheximide treated cells. The content of Alu sequences increased by 1.5-2.5 times, "classical" satellite DNA--by 5-7 times, alpha-satellite--by 3-5 times.

摘要

从小鼠HeLa细胞中分离出小的多分散环状DNA（spcDNA）。细胞用浓度为1和50微克/毫升的环己酰亚胺处理。梯度级分点印迹到硝酸纤维素滤膜上，并与不同的重复DNA杂交。对于用环己酰亚胺处理的细胞，spcDNA级分中的重复DNA序列库增加。Alu序列的含量增加了1.5至2.5倍，“经典”卫星DNA增加了5至7倍，α卫星增加了3至5倍。

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1
[Increase in the quantity of highly repetitive sequences in extrachromosomal circular DNAs under inhibition of translation by cycloheximide].[在环己酰亚胺抑制翻译作用下，染色体外环状DNA中高度重复序列数量的增加]
Biull Eksp Biol Med. 1991 May;111(5):530-2.
2
[Characterization of cloned alpha-satellite sequence from the extrachromosomal DNA of HeLa cell culture].[从HeLa细胞培养物的染色体外DNA克隆的α-卫星序列的特征分析]
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Sequence organization of repetitive sequences enriched in small polydisperse circular DNAs from HeLa cells.来自HeLa细胞的富含小多分散环状DNA的重复序列的序列组织。
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