来自小鼠3T6细胞的小多分散环状DNA的分子克隆与特性分析
Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells.
作者信息
Sunnerhagen P, Sjöberg R M, Karlsson A L, Lundh L, Bjursell G
出版信息
Nucleic Acids Res. 1986 Oct 24;14(20):7823-38. doi: 10.1093/nar/14.20.7823.
We have isolated, cloned and analyzed small polydisperse circular (spc) DNA from mouse 3T6 cells. The representation of highly repeated mouse genome sequence families in spcDNA has been examined, and the B1 repeat appears overrepresented in spcDNA by two criteria. The majority of spcDNA clones, however, is made out by as yet uncharacterized middle repetitive sequences. We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
我们从小鼠3T6细胞中分离、克隆并分析了小多分散环状（spc）DNA。已检测了spcDNA中高度重复的小鼠基因组序列家族的代表性，根据两个标准，B1重复序列在spcDNA中似乎过度存在。然而，大多数spcDNA克隆是由尚未表征的中等重复序列组成。我们研究了用环己酰亚胺处理单个序列后spcDNA群体的增加情况，发现这些序列会有差异地扩增。
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