Bouza C, Hermida M, Millán A, Vilas R, Vera M, Fernández C, Calaza M, Pardo B G, Martínez P
Departamento de Xenética, Facultade de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
Anim Genet. 2008 Dec;39(6):666-70. doi: 10.1111/j.1365-2052.2008.01784.x. Epub 2008 Sep 11.
The detection of microsatellite sequences within expressed sequence tags (ESTs) connects potential markers with specific genes, generating type I markers. We have developed and mapped by linkage analysis a set of EST-derived microsatellites in the turbot, Scophthalmus maximus. One hundred and ninety-one microsatellites were identified from 9256 turbot ESTs. Primer design was possible with 98 microsatellites. After genotyping 25 wild turbot and the parents of two reference families for linkage analysis, 43 EST-derived microsatellites were selected because they met technical and polymorphism criteria. A final set of 31 EST-derived microsatellites could be mapped to 17 linkage groups of the turbot consensus map based on 242 anonymous microsatellites. Twenty-four microsatellite-containing ESTs were functionally annotated, confirming them as type I markers. Nineteen were mapped in the turbot consensus map. These EST-derived microsatellites constitute useful tools for genome scanning of turbot populations, marker-assisted selection programmes and comparative mapping.
在表达序列标签（ESTs）中检测微卫星序列可将潜在标记与特定基因联系起来，从而产生I型标记。我们通过连锁分析开发并定位了一组大菱鲆（Scophthalmus maximus）中源自EST的微卫星。从9256个大菱鲆EST中鉴定出191个微卫星。98个微卫星可进行引物设计。在对25条野生大菱鲆以及两个用于连锁分析的参考家系的亲本进行基因分型后，选择了43个源自EST的微卫星，因为它们符合技术和多态性标准。基于242个匿名微卫星，最终一组31个源自EST的微卫星可定位到大菱鲆一致性图谱的17个连锁群上。24个含微卫星的EST进行了功能注释，证实它们为I型标记。其中19个定位在大菱鲆一致性图谱中。这些源自EST的微卫星构成了用于大菱鲆群体基因组扫描、标记辅助选择计划和比较作图的有用工具。
