Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.
J Fish Biol. 2010 Apr;76(5):1190-204. doi: 10.1111/j.1095-8649.2010.02592.x.
Three normalized cDNA libraries were constructed, two of which were constructed from reproductive tissues ovary and testis, and the other one from pooled immune tissues including head kidney, intestine, liver and spleen. A total of 10 542 clones were sequenced generating 10 128 expressed sequence tags (ESTs). Cluster analysis indicated a total of 5808 unique sequences including 1712 contigs and 4096 singletons. A total of 4249 (73%) of the unique ESTs had significant hits to the non-redundant protein database, 2253 of which were annotated using Gene Ontology (GO) terms. A total of 311 microsatellites (with 246 having sufficient flanking sequences for primer design) and 6294 putative SNPs were identified. These genome resources provide the material basis for future microarray development, marker validation and genetic linkage and QTL analysis.
构建了三个标准化的 cDNA 文库，其中两个文库构建自生殖组织卵巢和睾丸，另一个文库构建自包括头肾、肠、肝和脾在内的混合免疫组织。总共对 10542 个克隆进行了测序，产生了 10128 个表达序列标签 (EST)。聚类分析表明，总共存在 5808 个独特序列，包括 1712 个重叠群和 4096 个单序列。独特 EST 中有 4249 个（73%）与非冗余蛋白质数据库具有显著匹配，其中 2253 个使用基因本体论 (GO) 术语进行了注释。总共鉴定出 311 个微卫星（其中 246 个具有足够的侧翼序列用于引物设计）和 6294 个假定的 SNP。这些基因组资源为未来的微阵列开发、标记验证以及遗传连锁和 QTL 分析提供了物质基础。
