Komabayashi T, Nonomura G, Watanabe L G, Marshall G W, Marshall S J
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, 707 Parnassus Avenue, D2246, University of California, San Francisco, CA 94143-0758, United States.
J Dent. 2008 Nov;36(11):953-8. doi: 10.1016/j.jdent.2008.08.002. Epub 2008 Sep 10.
To evaluate dentin tubule numerical density variations below the CEJ.
Three human non-carious permanent canines were sectioned parallel to the CEJ to obtain dentin disks 1mm thick whose surfaces were 1mm and 2mm below the CEJ. Each disk was sectioned into quarters resulting in four segment locations: facial, lingual, mesial, and distal. The outer (PDL side) and inner (pulp side) surfaces of the specimens were shaped to expose dentin with SiC papers and polished. Numerical tubule density was determined from SEM images. All data were statistically analyzed using a three-way ANOVA.
The dentin tubule density (number/mm(2)) ranged from 13,700 to 32,300. Dentin tubule density was relatively uniform at 1mm and 2mm below the CEJ and increased by a factor of about two from the outer to the inner surface, which was significantly different (P<0.0001).
The tubule density variations at the cervical root did not present marked.
评估牙骨质界（CEJ）下方牙本质小管的数量密度变化。
将三颗人类非龋性恒牙犬齿沿与CEJ平行的方向切片，以获得厚度为1mm的牙本质盘，其表面位于CEJ下方1mm和2mm处。每个牙本质盘被切成四等份，形成四个切片位置：唇面、舌面、近中面和远中面。使用碳化硅砂纸对标本的外表面（牙周膜侧）和内表面（牙髓侧）进行修整，以暴露牙本质并进行抛光。通过扫描电子显微镜（SEM）图像确定牙本质小管的数量密度。所有数据均使用三因素方差分析进行统计分析。
牙本质小管密度（每平方毫米的数量）范围为13,700至32,300。在CEJ下方1mm和2mm处，牙本质小管密度相对均匀，从外表面到内表面增加了约两倍，差异具有统计学意义（P<0.0001）。
颈根部的牙本质小管密度变化不明显。
