Nogueira Amanda Palmeira Arruda, Grazziotin-Soares Renata, Leal Adriana Marques Mesquita, Freitas Júnior Sérgio Alves Guida, Gonçalves Bruna Laís Lins, Bauer José, Ferreira Meire Coelho, Carvalho Ceci Nunes
School of Dentistry, CEUMA University, São Luís 65075-120, MA, Brazil.
Department of Oral Biological Medical Sciences, Faculty of Dentistry, University of British Columbia UBC, Vancouver, BC V6T 1Z4, Canada.
Dent J (Basel). 2024 Jun 29;12(7):201. doi: 10.3390/dj12070201.
Antibiotic pastes used as intracanal medication in cases of revascularization therapy might cause negative effects on tooth properties, such as a reduction in dentin microhardness. This in vitro study investigated dentin microhardness in three different locations distancing from the canal lumen after 20 days of treatment with a tri-antibiotic paste (ciprofloxacin, metronidazole, and minocycline), and with a double-antibiotic paste (ciprofloxacin and metronidazole), with calcium hydroxide [Ca(OH)] Ultracal XS-treated dentin as comparison. Human mandibular premolars (n = 48) had the root canals cleaned and shaped and were used to produce dentin slices. Dentin slices remained immersed in the medications for 20 days. The Knoop microhardness (KHN) test was performed before (baseline/Day-0) and after treatment (Day-20) with the medications. Indentations were made at 25 µm, 50 µm, and 100 µm distances from the root canal lumen. The KHN was compared intra-group using Wilcoxon's test. Independent groups were compared using Mann-Whitney's and Kruskal-Wallis' tests, at α = 5%. The microhardness in all the tested groups was reduced at Day-20 in comparison with Day-0 ( < 0.001) (intra-group comparison/same distances). The Day-0 values were similar, and the Day-20 values were higher for the Ca(OH) group ( < 0.05) (comparison between groups/same distances). Calcium hydroxide for 20 days would be preferred rather than antibiotic pastes to minimize the expected reduction in dentin microhardness during regenerative procedures.
在牙髓血管再生治疗中用作根管内药物的抗生素糊剂可能会对牙齿性能产生负面影响，例如牙本质显微硬度降低。这项体外研究调查了用三联抗生素糊剂（环丙沙星、甲硝唑和米诺环素）和双联抗生素糊剂（环丙沙星和甲硝唑）治疗20天后，与用氢氧化钙[Ca(OH)] Ultracal XS处理的牙本质相比，距根管腔不同距离的三个不同位置的牙本质显微硬度。选取48颗人下颌前磨牙，对根管进行清理和塑形，制作牙本质切片。牙本质切片在药物中浸泡20天。在用药前（基线/第0天）和用药后（第20天）进行努氏显微硬度（KHN）测试。在距根管腔25 µm、50 µm和100 µm处进行压痕。组内比较采用Wilcoxon检验。独立组间比较采用Mann-Whitney检验和Kruskal-Wallis检验，α = 5%。与第0天相比，所有测试组在第20天的显微硬度均降低（<0.001）（组内比较/相同距离）。第0天的值相似，Ca(OH)组在第20天的值更高（<0.05）（组间比较/相同距离）。在再生过程中，为尽量减少预期的牙本质显微硬度降低，使用20天的氢氧化钙优于抗生素糊剂。
