Egyptian Russian University, Badr city, Egypt.
Faculty of Dentistry, Minia University, Minia, Egypt.
BMC Oral Health. 2023 Jun 9;23(1):377. doi: 10.1186/s12903-023-02995-z.
This study aimed to evaluate the effect of various irrigation protocols on the penetration depth of a calcium silicate-based sealer into dentinal tubules using confocal laser scanning microscopy (CLSM).
Twenty single-rooted mandibular premolars were endodontically prepared and divided into the following two groups according to the irrigation protocol used (n = 10): Group I: NaOCl + EDTA and Group II: continuous chelation (NaOCl/Dual Rinse). Obturation was performed with the warm vertical compaction technique using TotalFill HiFlow bioceramic sealer mixed with a fluorophore dye. Samples were observed using CLSM at 10× to measure the percentage of sealer penetration and its maximum depth into the dentinal tubules. Data were analysed using one-way ANOVA followed by Tukey's post-hoc test. The significance level was set at p < 0.05 within all tests.
Comparing the overall results of all sections tested, no statistically significant differences existed between the groups regarding the percentage of sealer penetration (p = 0.612) and maximum depth of penetration (p > 0.05).
With both types of irrigation used, dentinal tubule penetration was higher in the coronal section than in the apical section. Continuous chelation using NaOCl/Dual Rinse HEDP performed better in the coronal segments, while irrigation using NaOCl + EDTA promoted a higher percentage of sealer penetration in the apical segment.
本研究旨在使用共聚焦激光扫描显微镜（CLSM）评估不同冲洗方案对钙硅酸钠基封闭剂进入牙本质小管的渗透深度的影响。
20 颗下颌单根前磨牙根据所使用的冲洗方案（n=10）分为两组：I 组：次氯酸钠+EDTA；II 组：连续螯合（次氯酸钠/双重冲洗）。使用 TotalFill HiFlow 生物陶瓷封闭剂混合荧光染料，采用温热垂直加压技术进行根管充填。使用 CLSM 在 10×下观察样本，以测量封闭剂渗透的百分比及其进入牙本质小管的最大深度。使用单因素方差分析（ANOVA）后进行 Tukey 事后检验对数据进行分析。所有测试的显著水平均设为 p<0.05。
比较所有测试节段的总体结果，两组间在封闭剂渗透百分比（p=0.612）和最大渗透深度（p>0.05）方面均无统计学差异。
使用两种冲洗方式，冠部的牙本质小管渗透高于根尖部。使用 NaOCl/Dual Rinse HEDP 进行连续螯合在冠段表现更好，而使用 NaOCl+EDTA 冲洗在根尖段促进了更高百分比的封闭剂渗透。
