Sunada Yosuke, Nakamura Syoji, Kamei Chiaki
Fundamental Laboratory, Ohayo Dairy Products Co., Ltd., Okayama 701-1221, Japan.
Int Immunopharmacol. 2008 Dec 20;8(13-14):1761-6. doi: 10.1016/j.intimp.2008.08.011. Epub 2008 Oct 5.
In this study, we examined whether Lactobacillus acidophilus strain L-55 (strain L-55) suppresses the development of atopic dermatitis (AD)-like skin lesions induced by repeated application of 2,4,6-trinitrochlorobenzene (TNCB) in sensitized NC/Nga mice. The effect of strain L-55 was assessed by measuring clinical symptoms, ear swelling, scratching behavior and serum levels of total IgE. Strain L-55 was administered orally once a day: Strain L-55 at doses of 1 and 10 mg cells/mouse inhibited the development of AD-like skin lesions in dermatitis scores for the back. The increase of dermatitis score and ear swelling was also inhibited by strain L-55. In addition, strain L-55 also caused an inhibition of histological changes induced by repeated application of TNCB. Scratching behavior observed in the back and ear was inhibited by strain L-55. Furthermore elevated serum IgE levels observed by TNCB were also decreased by strain L-55. These results indicate that the inhibition of strain L-55 on AD-like lesions induced by repeated application of TNCB in sensitized NC/Nga mice occurred via a decrease in the serum total IgE level.
在本研究中，我们检测了嗜酸乳杆菌L-55株（L-55株）是否能抑制在致敏的NC/Nga小鼠中反复涂抹2,4,6-三硝基氯苯（TNCB）诱导的特应性皮炎（AD）样皮肤损伤的发展。通过测量临床症状、耳部肿胀、搔抓行为和血清总IgE水平来评估L-55株的作用。L-55株每天口服给药一次：1和10mg菌体/小鼠剂量的L-55株抑制了背部皮炎评分中AD样皮肤损伤的发展。L-55株还抑制了皮炎评分和耳部肿胀的增加。此外，L-55株还抑制了反复涂抹TNCB诱导的组织学变化。L-55株抑制了在背部和耳部观察到的搔抓行为。此外，L-55株还降低了TNCB诱导的血清IgE水平升高。这些结果表明，L-55株对致敏的NC/Nga小鼠中反复涂抹TNCB诱导的AD样损伤的抑制作用是通过降低血清总IgE水平实现的。
