Kao T H, Huang S C, Inbaraj B Stephen, Chen B H
Department of Food Science, Fu Jen University, Taipei 242, Taiwan.
Anal Chim Acta. 2008 Sep 26;626(2):200-11. doi: 10.1016/j.aca.2008.07.049. Epub 2008 Aug 8.
Gynostemma pentaphyllum (Thunb.) Makino, a traditional Chinese herb possessing antitumor and antioxidant activities, has been shown to contain several functional components like saponins and flavonoids. However, their identities remain uncertain. The objectives of this study were to develop an appropriate extraction, purification and HPLC-MS method to determine saponins and flavonoids in G. pentaphyllum. Both flavonoids and saponins were extracted with methanol, followed by purification with a C18 cartridge to elute the former with 50% methanol and the latter with 100% methanol. A total of 34 saponins were separated within 40 min by a Gemini C18 column and a gradient mobile phase of acetonitrile and 0.1% formic acid in water, in which 18 saponins were identified by LC-MS with ESI mode and Q-TOF (LC/MS/MS). Similarly, a total of eight flavonoids were separated within 45 min by the same column and a gradient solvent system of methanol and 0.1% formic acid in water, with identification being carried out by a post-column derivatization method and LC-MS with ESI mode. The amounts of flavonoids in G. pentaphyllum ranged from 170.7 to 2416.5 mug g(-1), whereas saponins were from 491.0 to 89,888.9 mug g(-1).
绞股蓝（学名：Gynostemma pentaphyllum (Thunb.) Makino），一种具有抗肿瘤和抗氧化活性的传统中草药，已被证明含有多种功能成分，如皂苷和黄酮类化合物。然而，它们的具体成分仍不确定。本研究的目的是开发一种合适的提取、纯化和高效液相色谱-质谱联用方法，以测定绞股蓝中的皂苷和黄酮类化合物。黄酮类化合物和皂苷均用甲醇提取，然后用C18柱纯化，用50%甲醇洗脱前者，用100%甲醇洗脱后者。通过Gemini C18柱以及乙腈和0.1%甲酸水溶液的梯度流动相，在40分钟内分离出了总共34种皂苷，其中18种皂苷通过电喷雾电离（ESI）模式和串联四极杆飞行时间质谱（Q-TOF，LC/MS/MS）进行了鉴定。同样，通过同一根柱子以及甲醇和0.1%甲酸水溶液的梯度溶剂系统，在45分钟内分离出了总共8种黄酮类化合物，采用柱后衍生化方法和电喷雾电离（ESI）模式的液相色谱-质谱联用进行鉴定。绞股蓝中黄酮类化合物的含量在170.7至2416.5μg g-1之间，而皂苷的含量在491.0至89,888.9μg g-1之间。
