Hoffman Michael D, Sniatynski Matthew J, Kast Juergen
The Biomedical Research Centre, University of British Columbia, Vancouver, BC, Canada.
Anal Chim Acta. 2008 Oct 3;627(1):50-61. doi: 10.1016/j.aca.2008.03.032. Epub 2008 Mar 22.
More and more attention is being focused on the analysis of post-translational modifications (PTMs) on proteins as researchers are continually learning how essential they are for proper cellular function. As there are hundreds of different types of known PTMs, traditional methods of modification analysis are incapable of comprehensively monitoring for post-translational modifications, a task which is a necessity for truly understanding a cell's biology. This review highlights recent developments in novel multiplexed methods of PTM analysis including: fluorescent stain and immuno-based methods, hardware-based mass spectrometric methods and computational-based mass spectrometric methods. Many of these techniques show great promise and will likely be a valuable resource for the biological community.
随着研究人员不断了解蛋白质翻译后修饰（PTM）对细胞正常功能的重要性，越来越多的注意力集中在对其的分析上。由于已知有数百种不同类型的PTM，传统的修饰分析方法无法全面监测翻译后修饰，而这一任务对于真正理解细胞生物学来说是必不可少的。本综述重点介绍了PTM分析新型多重方法的最新进展，包括：荧光染色和基于免疫的方法、基于硬件的质谱方法和基于计算的质谱方法。这些技术中的许多都显示出巨大的潜力，可能会成为生物学界的宝贵资源。
