Kobayashi Y, Akamatsu Y, Iwane T, Nakamura A, Satomi S
Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
Transplant Proc. 2009 Jan-Feb;41(1):49-51. doi: 10.1016/j.transproceed.2008.09.055.
We have previously reported that oxygenated warm perfusion prior to cold preservation (preperfusion) improved the function and viability of liver grafts from non-heart-beating donors (NHBD) using an ex vivo perfusion model. In this study, we evaluated the signaling pathway underlying these effects as well as the additive effect of preperfusion administration of edaravone, a free radical scavenger. Preperfusion treatment suppressed activation of JNK, p38 MAPK, and ERK. The addition of edaravone provided an insignificant increase in bile production and a trend to a decrease in TUNEL-positive cells. Oxygenated perfusion prior to cold preservation improved the function and viability of the grafts from NHBD, which accompanied impairment of MAPK activation. Moreover, the addition of edaravone significantly enhanced the effects of preperfusion.
我们之前曾报道，在冷保存之前进行氧合温灌注（预灌注），使用体外灌注模型可改善非心脏跳动供体（NHBD）肝脏移植物的功能和活力。在本研究中，我们评估了这些效应背后的信号通路以及自由基清除剂依达拉奉预灌注给药的附加效应。预灌注处理抑制了JNK、p38 MAPK和ERK的激活。添加依达拉奉使胆汁分泌略有增加，并使TUNEL阳性细胞有减少趋势。冷保存前的氧合灌注改善了NHBD移植物的功能和活力，这伴随着MAPK激活的受损。此外，添加依达拉奉显著增强了预灌注的效果。
