Jia Fei, Shan Changsheng, Li Fenghua, Niu Li
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Biosens Bioelectron. 2008 Dec 1;24(4):951-6. doi: 10.1016/j.bios.2008.07.057. Epub 2008 Aug 5.
A novel glucose biosensor based on immobilization of glucose oxidase (GOD) in thin films of polyethylenimine-functionalized ionic liquid (PFIL), containing a mixture of carbon nanotubes (CNT) and gold nanoparticles (AuNPs) and deposited on glassy carbon electrodes, was developed. Direct electrochemistry of glucose oxidase in the film was observed, with linear glucose response up to 12mM. The PFIL-stabilized gold nanoparticles had a diameter of 2.4+/-0.8nm and exhibited favorable stability (stored even over one month with invisible change in UV-vis spectroscopic measurements). In addition, CNT were also well dispersed in the PFIL matrix, then, the resulting CNT/AuNPs/PFIL composites film showed high electrocatalytic activity toward reduction of hydrogen peroxide and oxygen. Here, PFIL, due to its high ionic conductivity, good solubility to CNT, and stability to nanoparticles, played an important role in constructing stable CNT/AuNPs/PFIL/GOD composites. And good biocompatibility of PFIL also offered a friendly environment for the immobilization of biomolecules.
开发了一种新型葡萄糖生物传感器，该传感器基于将葡萄糖氧化酶（GOD）固定在聚乙烯亚胺功能化离子液体（PFIL）薄膜中，该薄膜含有碳纳米管（CNT）和金纳米颗粒（AuNP）的混合物，并沉积在玻碳电极上。观察到薄膜中葡萄糖氧化酶的直接电化学，对葡萄糖的线性响应高达12mM。PFIL稳定的金纳米颗粒直径为2.4±0.8nm，具有良好的稳定性（即使储存一个多月，紫外可见光谱测量中也无明显变化）。此外，CNT也很好地分散在PFIL基质中，因此，所得的CNT/AuNPs/PFIL复合膜对过氧化氢和氧气的还原表现出高电催化活性。在此，PFIL由于其高离子导电性、对CNT的良好溶解性以及对纳米颗粒的稳定性，在构建稳定的CNT/AuNPs/PFIL/GOD复合材料中发挥了重要作用。PFIL良好的生物相容性也为生物分子的固定提供了友好的环境。
