State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Lushan Road, Changsha 410082, PR China.
Talanta. 2010 Mar 15;80(5):1719-24. doi: 10.1016/j.talanta.2009.10.012. Epub 2009 Oct 14.
Polymerized ionic liquid-wrapped carbon nanotubes (PIL-CNTs) were firstly designed for direct electrochemistry and biosensing of redox proteins. The CNTs were coated successfully with polymerized ionic liquid (PIL) layer, as verified by transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infrared (FT-IR) spectroscopy. The PIL-CNTs were dispersed better in water and showed superior electrocatalysis toward O(2) and H(2)O(2) comparing to pristine CNTs and the mixture of IL monomer and CNTs. With glucose oxidase (GOD) as a protein model, the direct electrochemistry of the redox protein was investigated on the PIL-CNTs modified glassy carbon (GC) electrode and excellent direct electrochemical performance of GOD molecules was observed. The proposed biosensor (GOD/PIL-CNTs/GC electrode) displayed good analytical performance for glucose with linear response up to 6mM, response sensitivity of 0.853 microA mM(-1), good stability and selectivity.
聚合离子液体包裹的碳纳米管（PIL-CNTs）首次被设计用于氧化还原蛋白的直接电化学和生物传感。通过透射电子显微镜（TEM）、热重分析（TGA）和傅里叶变换红外（FT-IR）光谱证实，成功地在 CNTs 上包覆了聚合离子液体（PIL）层。与原始 CNTs 和 IL 单体与 CNTs 的混合物相比，PIL-CNTs 在水中分散得更好，对 O(2) 和 H(2)O(2)的电催化性能更优。以葡萄糖氧化酶（GOD）作为蛋白质模型，在 PIL-CNTs 修饰的玻碳（GC）电极上研究了氧化还原蛋白的直接电化学，观察到 GOD 分子具有优异的直接电化学性能。所提出的生物传感器（GOD/PIL-CNTs/GC 电极）对葡萄糖具有良好的分析性能，线性响应高达 6mM，响应灵敏度为 0.853 μA mM(-1)，具有良好的稳定性和选择性。
