对博来霉素抗性基因进行宏基因组筛选。
Metagenomic screening for bleomycin resistance genes.
作者信息
Mori Toshio, Mizuta Shiori, Suenaga Hikaru, Miyazaki Kentaro
机构信息
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan.
出版信息
Appl Environ Microbiol. 2008 Nov;74(21):6803-5. doi: 10.1128/AEM.00873-08. Epub 2008 Sep 12.
Abstract
A metagenomic library of activated sludge was screened for bleomycin resistance genes. Two genes were identified that differed greatly from each other, from the genes of bleomycin-producing actinomycetes, and from those of clinical isolates. Therefore, the nonclinical environment is a rich reservoir of new resistance elements, and metagenomics can be used to sample the resistome rapidly.
摘要
对活性污泥的宏基因组文库进行了博来霉素抗性基因筛选。鉴定出两个基因，它们彼此之间、与产生博来霉素的放线菌的基因以及临床分离株的基因都有很大差异。因此，非临床环境是新抗性元件的丰富储存库，宏基因组学可用于快速对耐药基因组进行采样。
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