Treissman D, Smith G N, Patrick J, Brien J F
Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's University, Kingston, Ont., Canada.
Dev Pharmacol Ther. 1991;16(1):48-52.
The effect of in vitro incubation with ethanol (10-80 mM) on 15-hydroxyprostaglandin dehydrogenase (15-OH-PGDH) activity was determined in the brain stem (pons and medulla) of the fetal sheep at a mean gestational age of 126 days (term: about 147 days). Enzyme activity was determined by measuring the rate of oxidation of PGE2 to 15-keto-PGE2 using a radiometric assay. There was no statistically significant ethanol-induced inhibition of 15-OH-PGDH activity. The data apparently do not support the hypothesis that acute in vitro exposure to ethanol directly inhibits 15-OH-PGDH activity in the near-term fetal brain stem.
在平均妊娠龄为126天（足月约147天）的胎羊脑干（脑桥和延髓）中，测定了乙醇（10 - 80 mM）体外孵育对15 - 羟基前列腺素脱氢酶（15 - OH - PGDH）活性的影响。使用放射性测定法通过测量PGE2氧化为15 - 酮 - PGE2的速率来确定酶活性。乙醇诱导的对15 - OH - PGDH活性的抑制无统计学意义。这些数据显然不支持急性体外暴露于乙醇直接抑制近期胎羊脑干中15 - OH - PGDH活性这一假说。
