Coco B J, Bagg J, Cross L J, Jose A, Cross J, Ramage G
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, Scotland, UK.
Oral Microbiol Immunol. 2008 Oct;23(5):377-83. doi: 10.1111/j.1399-302X.2008.00439.x.
Oral yeasts are an important component of the resident microbial ecology of the oral cavity, but they are also associated with various forms of oral candidosis, such as denture stomatitis. Although Candida albicans is the predominant oral fungal pathogen, other species may also play an integral role in pathogenesis. The aim of this study was to examine the mycological ecology in patients with denture stomatitis, using an improved sampling technique, to determine whether species diversity and species quantity were related to oral pathology.
Thirty-seven patients attending the Glasgow Dental Hospital were enrolled in this study following informed consent. A full clinical history was obtained, including details of their oral hygiene practices and the levels of erythema based on Newton's classification scale. Oral rinse, denture sonicate, and swab samples were taken, which were processed for quantitative and qualitative analysis of oral yeasts.
The proportion of patients with no inflammation or Newton's Types I, II, and III were 31, 33, 25, and 14%, respectively. Denture sonication was a superior sampling procedure, with statistically greater quantities of yeasts isolated using this methodology (P < 0.01). The predominant oral yeasts isolated were C. albicans (75%) and Candida glabrata (30%), which were isolated in higher proportions in patients with the highest grades of inflammation (100 and 80%), and in combination from 80% of these patients.
This study has demonstrated that mixed C. albicans and C. glabrata biofilms may play an important role in the pathogenesis associated with severe inflammation in denture wearers.
口腔酵母菌是口腔常驻微生物生态的重要组成部分，但它们也与各种形式的口腔念珠菌病有关，如义齿性口炎。虽然白色念珠菌是主要的口腔真菌病原体，但其他菌种在发病机制中也可能发挥重要作用。本研究的目的是使用改进的采样技术检查义齿性口炎患者的真菌生态，以确定菌种多样性和菌种数量是否与口腔病理学有关。
37名就诊于格拉斯哥牙科医院的患者在获得知情同意后纳入本研究。获取了完整的临床病史，包括他们的口腔卫生习惯细节以及基于牛顿分类量表的红斑水平。采集了口腔冲洗液、义齿超声清洗液和拭子样本，对其进行处理以对口腔酵母菌进行定量和定性分析。
无炎症或牛顿I、II和III型的患者比例分别为31%、33%、25%和14%。义齿超声清洗是一种更好的采样方法，使用该方法分离出的酵母菌数量在统计学上显著更多（P < 0.01）。分离出的主要口腔酵母菌是白色念珠菌（75%）和光滑念珠菌（30%），在炎症程度最高的患者中分离比例更高（分别为100%和80%），并且在80%的此类患者中同时分离到这两种菌。
本研究表明，白色念珠菌和光滑念珠菌混合生物膜可能在义齿佩戴者严重炎症相关的发病机制中起重要作用。
