系统性红斑狼疮患者中干扰素诱导趋化因子转录水平升高与疾病活动及器官损伤的关联。

Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients.

作者信息

Fu Qiong, Chen Xiaoqing, Cui Huijuan, Guo Yanzhi, Chen Jing, Shen Nan, Bao Chunde

机构信息

Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shan Dong Middle Road, Shanghai 200001, PR China.

出版信息

Arthritis Res Ther. 2008;10(5):R112. doi: 10.1186/ar2510. Epub 2008 Sep 15.

DOI:10.1186/ar2510

PMID:18793417

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2592795/

Abstract

INTRODUCTION

Systemic lupus erythematosus (SLE) is a multi-system autoimmune disease with a heterogeneous course and varying degrees of severity and organ damage; thus, there is increasing interest in identifying biomarkers for SLE. In this study we correlated the combined expression level of multiple interferon-inducible chemokines with disease activity, degree of organ damage and clinical features in SLE, and we investigated their roles as biomarkers.

METHODS

Peripheral blood cells obtained from 67 patients with SLE patients, 20 patients with rheumatoid arthritis (RA) and 23 healthy donors were subjected to real-time PCR in order to measure the transcriptional levels of seven interferon-inducible chemokines (RANTES, MCP-1, CCL19, MIG, IP-10, CXCL11, and IL-8). The data were used to calculate a chemokine score for each participant, after which comparisons were performed between various groups of SLE patients and control individuals.

RESULTS

Chemokine scores were significantly elevated in SLE patients versus RA patients and healthy donors (P = 0.012 and P = 0.002, respectively). Chemokine scores were correlated positively with SLE Disease Activity Index 2000 scores (P = 0.005) and negatively with C3 levels (P < 0.001). Compared with patients without lupus nephritis and those with inactive lupus nephritis, chemokine scores were elevated in patients with active lupus nephritis, especially when their daily prednisone dosage was under 30 mg (P = 0.002 and P = 0.014, respectively). Elevated chemokine scores were also associated with the presence of cumulative organ damage (Systemic Lupus International Collaborating Clinics/American Society of Rheumatology Damage Index >or= 1; P = 0.010) and the occurrence of anti-Sm or anti-RNP autoantibodies (both P = 0.021).

CONCLUSIONS

The combined transcription level of interferon-inducible chemokines in peripheral blood leucocytes is closely associated with disease activity, degree of organ damage, and specific autoantibody patterns in SLE. The chemokine score may serve as a new biomarker for active and severe disease in SLE.

摘要

引言

系统性红斑狼疮（SLE）是一种多系统自身免疫性疾病，病程异质性，严重程度和器官损害程度各不相同；因此，人们对确定SLE的生物标志物越来越感兴趣。在本研究中，我们将多种干扰素诱导趋化因子的联合表达水平与SLE的疾病活动度、器官损害程度及临床特征进行关联，并研究它们作为生物标志物的作用。

方法

从67例SLE患者、20例类风湿关节炎（RA）患者和23名健康供者获取外周血细胞，进行实时PCR以测量7种干扰素诱导趋化因子（RANTES、MCP-1、CCL19、MIG、IP-10、CXCL11和IL-8）的转录水平。数据用于计算每位参与者的趋化因子评分，之后在不同组的SLE患者与对照个体之间进行比较。

结果

与RA患者和健康供者相比，SLE患者的趋化因子评分显著升高（分别为P = 0.012和P = 0.002）。趋化因子评分与2000年SLE疾病活动指数评分呈正相关（P = 0.005），与C3水平呈负相关（P < 0.001）。与无狼疮肾炎患者及狼疮肾炎非活动期患者相比，活动期狼疮肾炎患者的趋化因子评分升高，尤其是当他们每日泼尼松剂量低于30 mg时（分别为P = 0.002和P = 0.014）。趋化因子评分升高还与累积器官损害的存在（系统性红斑狼疮国际协作临床/美国风湿病学会损伤指数≥1；P = 0.010）以及抗Sm或抗RNP自身抗体的出现相关（两者P = 0.021）。