Chen Yixin, Xu Feihai, Fan Xiaohui, Luo Haifeng, Ge Shengxiang, Zheng Qingbing, Xia Ningshao, Chen Honglin, Guan Yi, Zhang Jun
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, The Key Laboratory of Education Minister for Cell Biology and Tumor Cell Engineering of Xiamen University, School of Life Science, Xiamen University, Xiamen 361005, China.
J Virol Methods. 2008 Dec;154(1-2):213-5. doi: 10.1016/j.jviromet.2008.08.013. Epub 2008 Sep 30.
The performance of H5 Dot ELISA, a rapid test for detection of avian H5N1 influenza virus, was evaluated using 30 H5N1 strains belonging to 10 major genetic groups of H5N1 influenza virus, 14 strains of non-H5N1 influenza virus and 652 field samples collected from healthy and diseased chickens from markets and poultry farms. The detection limit of the test for all 30 strains of H5N1 virus was < or = 0.1 hemagglutinin (HA) units and the test yielded a negative result when tested against 100 HA units of the non-H5N1 viruses. The test gave a positive result for 87 of the 106 poultry samples from which H5N1 virus was isolated by culture and 3 of 546 culture-negative poultry samples. Compared with virus culture, the overall prediction rate of the test was determined to be 96.6%; the positive prediction rate was 96.7% and negative prediction rate, 96.6%. The false positive rate was 0.5% and false negative rate 17.9%. Considering that the test is also convenient to use, it was concluded that H5 Dot ELISA is suitable for field use in the investigation of H5N1 influenza outbreaks and surveillance in poultry.
利用属于H5N1流感病毒10个主要基因群的30株H5N1毒株、14株非H5N1流感病毒毒株以及从市场和家禽养殖场的健康与患病鸡采集的652份现场样本，对用于检测禽H5N1流感病毒的快速检测方法H5斑点酶联免疫吸附测定（H5 Dot ELISA）的性能进行了评估。该检测方法对所有30株H5N1病毒的检测限均≤0.1血凝素（HA）单位，在用100 HA单位的非H5N1病毒进行检测时，该检测方法给出阴性结果。在通过培养分离出H5N1病毒的106份家禽样本中，该检测方法对其中87份给出阳性结果，在546份培养阴性的家禽样本中，该检测方法对其中3份给出阳性结果。与病毒培养相比，该检测方法的总体预测率为96.6%；阳性预测率为96.7%，阴性预测率为96.6%。假阳性率为0.5%，假阴性率为17.9%。鉴于该检测方法使用也方便，得出结论：H5 Dot ELISA适用于在H5N1流感疫情调查和家禽监测的现场使用。
