Platt D J, Smith I
University Department of Bacteriology, Royal Infirmary, Glasgow, Scotland.
Epidemiol Infect. 1991 Aug;107(1):213-23. doi: 10.1017/s0950268800048834.
As part of the investigation of a putative bovine outbreak, 13 isolates of Salmonella typhimurium phage type 204c were subjected to plasmid analysis. Plasmid profiles suggested that several distinct strains were involved and these observations were supported by minor variations in antibiotic resistance pattern. Restriction enzyme fingerprinting and conjugational segregation of the plasmids confirmed these findings. Although 12 of the 13 isolates were resistant to gentamicin, resistance was conferred by 4 distinct plasmids; 3 of these belonged to Inc I and were distantly related on the basis of restriction fingerprints and the fourth was a resistant derivative of the 60 MDa S. typhimurium serotype-specific plasmid. The molecular evidence refuted the hypothesis that geographical and temporal clustering of these gentamicin-resistant isolates could be explained on the basis of a single epidmiological episode.
作为对一起疑似牛群疫情调查的一部分，对13株鼠伤寒沙门氏菌204c型噬菌体进行了质粒分析。质粒图谱表明涉及几种不同的菌株，抗生素抗性模式的微小差异也支持了这些观察结果。质粒的限制性酶切指纹分析和接合分离证实了这些发现。虽然13株分离株中有12株对庆大霉素耐药，但耐药性由4种不同的质粒赋予；其中3种属于Inc I型，根据限制性指纹图谱关系较远，第4种是60 MDa鼠伤寒沙门氏菌血清型特异性质粒的耐药衍生物。分子证据驳斥了这样一种假设，即这些庆大霉素耐药分离株在地理和时间上的聚集可以用单一的流行病学事件来解释。
