Holmberg S D, Wachsmuth I K, Hickman-Brenner F W, Cohen M L
J Clin Microbiol. 1984 Feb;19(2):100-4. doi: 10.1128/jcm.19.2.100-104.1984.
We compared the phage types, antimicrobial resistance patterns, and plasmid profiles of 20 groups of isolates received at the Centers for Disease Control from Salmonella typhimurium outbreaks between 1975 and 1982 to determine the most useful laboratory method for identifying epidemiologically related isolates of S. typhimurium. In 18 (90%) of the 20 outbreaks, epidemiologically related isolates were identified as being the same by each of the three methods. In a subgroup of nine outbreaks in which isolates unrelated to the outbreak were submitted for comparison, outbreak isolates were differentiated from such control isolates six times (67%) by phage typing alone, four times (44%) by antimicrobial susceptibility testing alone, and eight times (89%) by plasmid profile analysis alone. Epidemic isolates were multiply susceptible, nontypable, or without plasmids in 14 (70%), 1 (5%), and 3 (15%), respectively, of the 20 outbreaks. Plasmid analysis appeared to be at least as specific as phage typing in identifying epidemiologically related isolates of S. typhimurium as being the same or in differentiating them from control specimens; both techniques appeared to be superior to antimicrobial susceptibility testing.
我们比较了1975年至1982年间从鼠伤寒沙门氏菌疫情中收到的20组分离株的噬菌体类型、抗菌药物耐药模式和质粒图谱，以确定鉴定鼠伤寒沙门氏菌流行病学相关分离株最有用的实验室方法。在20起疫情中的18起（90%）中，三种方法中的每一种都将流行病学相关分离株鉴定为相同。在一个包含9起疫情的亚组中，提交了与疫情无关的分离株进行比较，仅通过噬菌体分型就有6次（67%）将疫情分离株与对照分离株区分开来，仅通过抗菌药物敏感性测试有4次（44%）区分开来，仅通过质粒图谱分析有8次（89%）区分开来。在20起疫情中，分别有14起（70%）、1起（5%）和3起（15%）的流行分离株对多种药物敏感、不可分型或无质粒。在鉴定鼠伤寒沙门氏菌流行病学相关分离株是否相同或将其与对照样本区分开来方面，质粒分析似乎至少与噬菌体分型一样具有特异性；这两种技术似乎都优于抗菌药物敏感性测试。
