使用光开关增强型黄色荧光蛋白对新月柄杆菌活细胞进行超分辨率成像。
Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.
作者信息
Biteen Julie S, Thompson Michael A, Tselentis Nicole K, Bowman Grant R, Shapiro Lucy, Moerner W E
机构信息
Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.
出版信息
Nat Methods. 2008 Nov;5(11):947-9. doi: 10.1038/nmeth.1258. Epub 2008 Sep 15.
Abstract
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
摘要
常用的单体增强型黄色荧光蛋白（EYFP）能够对活细胞内超出光学分辨率极限的蛋白质结构进行成像（“超分辨率”成像）。通过结合单个EYFP融合蛋白的光诱导激活和延时成像，我们获得了新月柄杆菌活细胞中细菌肌动蛋白MreB丝状超结构的分辨率低于40纳米的图像。这些研究表明，EYFP是用于体内超分辨率成像的一种有用的荧光发射体。
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本文引用的文献
1
Proposed method for molecular optical imaging.分子光学成像的建议方法。
Opt Lett. 1995 Feb 1;20(3):237-9. doi: 10.1364/ol.20.000237.
2
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.纳米级黏附动力学的活细胞光活化定位显微镜技术
Nat Methods. 2008 May;5(5):417-23. doi: 10.1038/nmeth.1202. Epub 2008 Apr 13.
3
Investigating intracellular dynamics of FtsZ cytoskeleton with photoactivation single-molecule tracking.利用光激活单分子追踪技术研究FtsZ细胞骨架的细胞内动力学。
Biophys J. 2008 Aug;95(4):2009-16. doi: 10.1529/biophysj.108.128751. Epub 2008 Apr 4.
4
Getting organized--how bacterial cells move proteins and DNA.有条不紊——细菌细胞如何转运蛋白质和DNA。
Nat Rev Microbiol. 2008 Jan;6(1):28-40. doi: 10.1038/nrmicro1795.
5
Single-molecule mountains yield nanoscale cell images.单分子成像技术可生成纳米级细胞图像。
Nat Methods. 2006 Oct;3(10):781-2. doi: 10.1038/nmeth1006-781.
6
Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.通过荧光光激活定位显微镜进行的超高分辨率成像。
Biophys J. 2006 Dec 1;91(11):4258-72. doi: 10.1529/biophysj.106.091116. Epub 2006 Sep 15.
7
Imaging intracellular fluorescent proteins at nanometer resolution.以纳米分辨率成像细胞内荧光蛋白。
Science. 2006 Sep 15;313(5793):1642-5. doi: 10.1126/science.1127344. Epub 2006 Aug 10.
8
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).基于随机光学重建显微镜（STORM）的亚衍射极限成像
Nat Methods. 2006 Oct;3(10):793-5. doi: 10.1038/nmeth929. Epub 2006 Aug 9.
9
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10
An actin-like gene can determine cell polarity in bacteria.一个类似肌动蛋白的基因能够决定细菌中的细胞极性。
Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8643-8. doi: 10.1073/pnas.0402638101. Epub 2004 May 24.
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