Biteen Julie S, Thompson Michael A, Tselentis Nicole K, Bowman Grant R, Shapiro Lucy, Moerner W E
Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.
Nat Methods. 2008 Nov;5(11):947-9. doi: 10.1038/nmeth.1258. Epub 2008 Sep 15.
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
常用的单体增强型黄色荧光蛋白(EYFP)能够对活细胞内超出光学分辨率极限的蛋白质结构进行成像(“超分辨率”成像)。通过结合单个EYFP融合蛋白的光诱导激活和延时成像,我们获得了新月柄杆菌活细胞中细菌肌动蛋白MreB丝状超结构的分辨率低于40纳米的图像。这些研究表明,EYFP是用于体内超分辨率成像的一种有用的荧光发射体。