Rust Michael J, Bates Mark, Zhuang Xiaowei
Department of Physics, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Nat Methods. 2006 Oct;3(10):793-5. doi: 10.1038/nmeth929. Epub 2006 Aug 9.
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
我们基于可光开关荧光团的高精度定位开发了一种高分辨率荧光显微镜方法。在每个成像周期中，只有一小部分荧光团被开启，从而能够以纳米精度确定它们的位置。从一系列成像周期中获得的荧光团位置被用于重建整体图像。我们展示了20纳米的成像分辨率。从原理上讲，这项技术可以达到分子尺度的分辨率。
