Moorman A F, van den Hoff M J, de Boer P A, Charles R, Lamers W H
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
FEBS Lett. 1991 Aug 19;288(1-2):133-7. doi: 10.1016/0014-5793(91)81019-5.
A hybridocytochemical approach has been applied to establish whether the gene for the C/EBP mRNA might be involved in the topographical regulation of gene expression in adult rat liver. To that end the spatial distribution of the mRNA of C/EBP has been compared to that of the mRNAs of glutamine synthetase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glucokinase (GK) in normal adult livers, in livers from dexamethasone-treated animals and in livers from starved animals refed with glucose for 4 h. In normal rat liver, in situ hybridization with a probe for C/EBP mRNA revealed a low density of apparently homogeneously distributed grains, indicating low levels of C/EBP mRNA. In contrast, the livers of the experimentally-treated animals revealed a zonal distribution of the mRNA of C/EBP with the highest density of grains around the central venules. The dynamics of the pattern of expression of C/EBP mRNA are virtually identical to that of the GK mRNA. These data qualify C/EBP mRNA as a pericentral mRNA and suggest a role for the C/EBP protein in the topographical regulation of the expression of the GK mRNA.
已采用一种杂交细胞化学方法来确定C/EBP mRNA的基因是否可能参与成年大鼠肝脏基因表达的区域调节。为此，在正常成年肝脏、地塞米松处理动物的肝脏以及饥饿4小时后再喂食葡萄糖的动物肝脏中，将C/EBP的mRNA的空间分布与谷氨酰胺合成酶（GS）、磷酸烯醇丙酮酸羧激酶（PEPCK）和葡萄糖激酶（GK）的mRNA的空间分布进行了比较。在正常大鼠肝脏中，用C/EBP mRNA探针进行原位杂交显示，明显均匀分布的颗粒密度较低，表明C/EBP mRNA水平较低。相比之下，经实验处理的动物肝脏显示C/EBP mRNA呈区域分布，中央静脉周围颗粒密度最高。C/EBP mRNA表达模式的动态变化与GK mRNA的动态变化几乎相同。这些数据将C/EBP mRNA定性为中央周围mRNA，并提示C/EBP蛋白在GK mRNA表达的区域调节中发挥作用。
