Moorman A F, de Boer P A, Charles R, Lamers W H
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
FEBS Lett. 1991 Aug 5;287(1-2):47-52. doi: 10.1016/0014-5793(91)80013-s.
The spatial distribution of glucokinase mRNA (GK mRNA) in rat liver was studied by in situ hybridization under normal and inducing conditions. GK mRNA was first detectable in the liver parenchyma of neonatal rats of 1.5 days. The density of grains decreases in a central-portal direction. This pattern remains essentially unchanged up to 15 days, after which the adult type of distribution gradually starts to develop, i.e. low density of grains indicating low levels of GK mRNA, in which no gradient of expression could be visualized. Within 2 h after an oral glucose load to starved animals, the GK mRNA expression pattern changed from hardly detectable to a clear gradient with the highest grain density around the terminal central venules. Within 6 h relatively high levels of grains, almost homogeneously distributed across the liver lobule, were observed. Glucocorticosteroid treatment also induced GK mRNA in the pericentral area. It is concluded that the observed induction pattern qualifies GK mRNA as a pericentral mRNA suggesting that the pericentral expression pattern of the protein is primarily regulated at the pretranslational level.
通过原位杂交技术研究了正常和诱导条件下大鼠肝脏中葡萄糖激酶mRNA（GK mRNA）的空间分布。在1.5日龄新生大鼠的肝实质中首次检测到GK mRNA。颗粒密度沿中央-门静脉方向降低。这种模式在15天前基本保持不变，之后逐渐开始形成成年型分布，即颗粒密度低表明GK mRNA水平低，其中无法观察到表达梯度。饥饿动物口服葡萄糖负荷后2小时内，GK mRNA表达模式从几乎检测不到变为清晰的梯度，终末中央静脉周围颗粒密度最高。6小时内，观察到相对高水平的颗粒，几乎均匀分布于肝小叶。糖皮质激素处理也诱导中央周围区域的GK mRNA表达。得出的结论是，观察到的诱导模式使GK mRNA成为中央周围mRNA，这表明该蛋白质的中央周围表达模式主要在翻译前水平受到调节。
