Kano Y, Ogawa T, Ogura T, Hiraga S, Okazaki T, Imamoto F
Department of Molecular Genetics, Kyoto Pharmaceutical University, Japan.
Gene. 1991 Jul 15;103(1):25-30. doi: 10.1016/0378-1119(91)90386-p.
The closely related Escherichia coli genes, hupA, hupB, himA and himD (hip), encode the bacterial histone-like protein subunits, HU-2, HU-1, IHF chi and IHF beta, respectively. We report here that E. coli minichromosomes [plasmids (2.7-12.2 kb) with oriC] carrying the intact mioC region were unable to transform mutants deficient in both HU and integration host factor (IHF), whereas they could transform mutants deficient in either HU or IHF as efficiently as the wild-type strain. Minichromosomes carrying a deletion of the proximal part of mioC or a DnaA box just upstream from mioC could not transform cells deficient in IHF, but could transform cells deficient in HU. These results suggested that HU and IHF participate in minichromosomal replication from oriC in E. coli.
密切相关的大肠杆菌基因hupA、hupB、himA和himD（hip）分别编码细菌组蛋白样蛋白亚基HU-2、HU-1、整合宿主因子（IHF）χ和IHFβ。我们在此报告，携带完整mioC区域的大肠杆菌微型染色体[带有oriC的质粒（2.7 - 12.2 kb）]无法转化HU和整合宿主因子（IHF）均缺陷的突变体，而它们能够像野生型菌株一样高效地转化仅缺乏HU或IHF的突变体。携带mioC近端部分缺失或mioC上游一个DnaA框缺失的微型染色体无法转化缺乏IHF的细胞，但能够转化缺乏HU的细胞。这些结果表明，HU和IHF参与大肠杆菌中oriC介导的微型染色体复制。
