Ogura T, Niki H, Kano Y, Imamoto F, Hiraga S
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Mol Gen Genet. 1990 Jan;220(2):197-203. doi: 10.1007/BF00260482.
Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the delta hupA-delta hupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the delta hupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the delta hupA-delta hupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable.
互补和测序分析表明，无法支持微型F质粒稳定维持的hopD突变体（Niki等人，1988年）在hupB基因中存在突变，且hopD410突变是HU-1第5位谷氨酰胺位置的赭石型突变。在hupA和hupB突变体（HU突变体）以及himA和hip突变体（IHF突变体）中研究了各种质粒、微型P1质粒、微型F质粒和oriC质粒的维持和稳定性。微型P1质粒和微型F质粒无法导入ΔhupA-ΔhupB双缺失突变体。微型F质粒的复制在hupB突变体（包括ΔhupB和hopD（hupB）突变体）中受到部分抑制，而oriC质粒的复制即使在ΔhupA-ΔhupB双缺失突变体中也未受到显著影响。微型P1质粒在himA-hip突变体中略有不稳定，而微型F质粒则稳定。
