大鼠RBA骨髓性白血病长期培养物的生长特性和致瘤性
Growth characteristics and tumorigenicity of long-term cultures of the rat RBA myelogenous leukemia.
作者信息
Svec J, Hlavayová E, Thurzo V
出版信息
Neoplasma. 1976;23(6):609-16.
PMID:187974
Abstract
A continuous cell line, RBA-BC, has been derived from the buffy coat of heparinized blood of a Sprague--Dawley rat with developed RBA myeloblastosis. Separations of myeloblasts from the RBA-BC cell line was accomplished by velocity sedimentation in Verografin density gradients. The cells were monitored during long-term culture by generation time analysis. Cytochemical studies revealed the myelogenous type of RBA leukemia.
摘要
一种连续细胞系RBA - BC，是从患有RBA成髓细胞血症的斯普拉格 - 道利大鼠的肝素化血液的血沉棕黄层中分离出来的。通过在Verografin密度梯度中进行速度沉降，从RBA - BC细胞系中分离出成髓细胞。通过世代时间分析对长期培养过程中的细胞进行监测。细胞化学研究揭示了RBA白血病的髓细胞类型。
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