Martinez Carlos Augusto Real, Nonose Ronaldo, Spadari Ana Paula Pimentel, Máximo Felipe Rodrigues, Priolli Denise Gonçalves, Pereira José Aires, Margarido Nelson Fontana
São Francisco University Hospital, Brazil.
Acta Cir Bras. 2010 Jun;25(3):231-40. doi: 10.1590/s0102-86502010000300004.
To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion.
Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05).
There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion.
Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.
通过计算机辅助图像处理对结肠黏膜中硫黏液和涎黏液的表达强度进行量化，比较有和没有粪流的肠段，并与粪便转运排除持续时间进行关联分析。
45只Wistar大鼠通过构建近端结肠造口术和远端黏膜瘘使左半结肠粪流改道。它们被随机分为三个实验组，每组15只动物，其中10只接受结肠改道（实验亚组），5只仅接受剖腹手术，不进行结肠改道（对照亚组）。根据处死日期形成三个实验组，分别在手术后6周（A组）、12周（B组）和18周（C组）处死动物。采用高铁二胺-阿尔辛蓝（HID-AB）组织化学技术评估结肠黏膜中硫黏液和涎黏液的表达。借助计算机辅助图像分析软件，在两个随机视野中存在四个完整连续隐窝的位置，对每只动物有和没有粪流的肠段的组织表达进行量化。最终值取所选两个视野在有和没有粪流肠段中的平均读数。为比较有和没有粪流肠段中两种黏蛋白亚型的表达，采用配对学生t检验。根据排除持续时间分析方差，采用带有纽曼-基尔斯事后检验的方差分析，将显著性水平设定为5%（p<0.05）。
无论所考虑的排除持续时间如何，没有粪流的结肠组织中硫黏液和涎黏液含量均显著降低。随着排除持续时间增加，没有粪流的肠段组织中硫黏液含量增加，涎黏液含量降低。
粪流改道降低了没有粪流肠段的组织硫黏液和涎黏液含量。尽管没有粪流的肠段中两种酸性黏蛋白亚型水平均降低，但随着肠道改道持续时间增加，组织硫黏液含量增加，涎黏液含量降低。
