在动物模型中，结肠黏膜会受到十天胃限制的影响吗？

Is the colon mucosa affected by ten days of gastric restriction in an animal model?

作者信息

Akamatsu Flávia Emi, Fontes Luiz Gustavo, Itezerote Ana Maria, Saleh Samir, Teodoro Walcy Paganelli Rosolia, Artifon Everson, Hojaij Flávio, Andrade Mauro, Pereira José Aires, Martinez Carlos Augusto Real, Jacomo Alfredo Luiz

机构信息

PhD, Department of Surgery, Laboratory of Medical Research 02, Division of Human Structural Topography, Faculty of Medicine, Universidade de São Paulo (FMUSP), Brazil. Design, intellectual and scientific content of the study; manuscript writing.

Graduate student, Division of Human Structural Topography, FMUSP, Sao Paulo-SP, Brazil. Acquisition of data, technical procedures.

出版信息

Acta Cir Bras. 2019 Aug 19;34(6):e201900610. doi: 10.1590/s0102-865020190060000010.

DOI:10.1590/s0102-865020190060000010

PMID:31433001

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6705340/

Abstract

PURPOSE

To identify whether the colon mucosa is affected by ten days of gastric restriction in an animal model.

METHODS

An experimental model of gastric restriction was devised using rats. The animals were submitted to surgical gastrostomy, and a cylindrical loofah was inserted into the stomach. We studied 30 adult male Wistar rats divided into three groups: the stomach restriction group (R10); the sham group (S10), which underwent the same procedure except for the loofah insertion; and the control group (C10). The expression of neutral and acid mucins was evaluated using histochemical techniques. Goblet cells and protein content were compared between groups using generalized estimation equations (GEEs). Bonferroni's multiple comparison was applied to identify differences between the groups. All tests considered a 5% significance level.

RESULTS

There was an increased expression of neutral mucins, acid mucins and goblet cells in the R10 group. Collagen was also enhanced in the R10 group.

CONCLUSION

The colon mucosa is affected by ten days of gastric restriction in an animal model, increasing neutral mucins, acid mucins and collagen content with trophic maintenance.

摘要

目的

在动物模型中确定结肠黏膜是否受到十天胃限制的影响。

方法

使用大鼠设计了一种胃限制实验模型。对动物进行手术胃造口术，并将圆柱形丝瓜插入胃中。我们研究了30只成年雄性Wistar大鼠，分为三组：胃限制组（R10）；假手术组（S10），除未插入丝瓜外，进行相同手术；对照组（C10）。使用组织化学技术评估中性和酸性黏蛋白的表达。使用广义估计方程（GEEs）比较各组之间的杯状细胞和蛋白质含量。应用Bonferroni多重比较来确定组间差异。所有测试均采用5%的显著性水平。