Beltran S, Galinier R, Allienne J F, Boissier J
UMR 5244 CNRS-EPHE, Parasitologie Fonctionnelle et Evolutive, Biologie et Ecologie Tropical et Méditerranéene, Université de Perpignan, Perpignan, France.
Mem Inst Oswaldo Cruz. 2008 Aug;103(5):501-3. doi: 10.1590/s0074-02762008000500017.
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
血吸虫是一种体内寄生虫，可引发一种名为血吸虫病的严重人类疾病。对寄生虫遗传多样性进行量化是了解血吸虫病流行病学和疾病传播模式的重要组成部分。在本文中，我们提出了一种新颖的检测方法，用于快速、低成本且高效地提取曼氏血吸虫虫卵、幼虫和成虫阶段的DNA。一欧元就能以最快速度进行60,000次DNA提取反应（仅需15分钟孵育和5个操作步骤）。
