Herrmann-Hoesing Lynn M, White Stephen N, Mousel Michelle R, Lewis Gregory S, Knowles Donald P
US Department of Agriculture, Animal Disease Research Unit, Agricultural Research Service, Pullman, WA 99164-6630, USA.
Immunogenetics. 2008 Dec;60(12):749-58. doi: 10.1007/s00251-008-0328-9. Epub 2008 Sep 17.
Previous studies initiated defining the role of host genetics in influencing the outcome of exposure to ovine progressive pneumonia virus. However, specific genes influencing host control of virus replication and disease progression have not been identified. This study, using 383 ewes of the Columbia, Polypay, and Rambouillet breeds, tested the hypothesis that host control of OPPV as measured by provirus levels in the peripheral blood associates with certain breeds and MHC class II Ovis aries (Ovar)-DRB1 expressed alleles. Rambouillet ewes were less likely to have measurable provirus levels as compared to Columbia ewes at ages 5 and 6 (P value < 0.02), and they exhibited lower provirus levels when compared to both Columbia and Polypay ewes of the same ages (P value < 0.05). The presence of DRB10403- or DRB107012-expressed alleles were significantly associated (P value = 0.019 and 0.0002, respectively) with lower OPP provirus levels but only were only found in 11% of the ewe flock. Analysis of each segregating amino acid in the beta1 domain of DR beta-chain revealed that amino acids Y31, T32, N37, T51, Q60, or N74 significantly associated (P value range = 0.0003-0.018) with lower OPP provirus levels, whereas amino acids H32, A38, or I67 associated (P value range = 0.013-0.043) with higher OPP provirus levels. These results suggest that Ovar-DRB1 contributes as one host genetic factor that controls OPP provirus levels, but does not fully account for the breed-specific OPP proviral differences.
先前的研究开始界定宿主基因在影响绵羊进行性肺炎病毒暴露结果中的作用。然而，尚未确定影响宿主对病毒复制和疾病进展控制的特定基因。本研究使用了383只哥伦比亚、波利佩和兰布耶品种的母羊，检验了以下假设：以外周血前病毒水平衡量的宿主对绵羊进行性肺炎病毒（OPPV）的控制与某些品种以及表达的绵羊MHC II类（Ovar）-DRB1等位基因相关。与5岁和6岁的哥伦比亚母羊相比，兰布耶母羊出现可测量前病毒水平的可能性较小（P值<0.02），并且与相同年龄的哥伦比亚和波利佩母羊相比，它们的前病毒水平较低（P值<0.05）。表达DRB10403或DRB107012等位基因与较低的OPPV前病毒水平显著相关（P值分别为0.019和0.0002），但仅在11%的母羊群中发现。对DRβ链β1结构域中每个分离氨基酸的分析表明，氨基酸Y31、T32、N37、T51、Q60或N74与较低的OPPV前病毒水平显著相关（P值范围=0.0003 - 0.018），而氨基酸H32、A38或I67与较高的OPPV前病毒水平相关（P值范围=0.013 - 0.043）。这些结果表明，Ovar - DRB1作为控制OPPV前病毒水平的一个宿主遗传因素发挥作用，但不能完全解释品种特异性的OPPV前病毒差异。
