Ma Qing-Wen
Children's Hospital of Shanghai, Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China.
Yi Chuan. 2011 Jun;33(6):567-75. doi: 10.3724/sp.j.1005.2011.00567.
Integrase of phage fC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP. Meanwhile, this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes. Nowadays, fC31 integrase system is becoming a potential tool for genome modification, gene therapy and transgenic research. Recent progress of fC31 integrase system in integration mode in mammalian genomes, efficiency improvement and researches concerned on transgenic safety were summarized in this review.
噬菌体fC31的整合酶催化链霉菌附着位点attB与噬菌体附着位点attP之间的同源重组。同时，这种整合酶能够以位点特异性方式介导含attB的供体质粒整合到真核基因组中的假attP位点，从而使整合基因长期稳定表达。如今，fC31整合酶系统正成为基因组修饰、基因治疗及转基因研究的一种潜在工具。本文综述了fC31整合酶系统在哺乳动物基因组整合模式、效率提高及转基因安全性相关研究方面的最新进展。
