Sandt Christophe, Smith-Palmer Truis, Pink Judith, Pink David
Department of Chemistry, Centre for Applied Petroleum Sciences, St. Francis Xavier University, Antigonish, NS, Canada, B2G 2W5.
Appl Spectrosc. 2008 Sep;62(9):975-83. doi: 10.1366/000370208785793245.
The distribution of a carotene-containing yeast (CCY) in a biofilm formed by a small colony variant (SCV) of Pseudomonas aeruginosa PA01 was followed by confocal Raman microspectroscopy (CRM). SCV PA01 and CCY cells were distinguished by their spectral signatures, and the distribution of the overall biomass was monitored by the C-H bending or stretching signal. The distributions of total biomass, PA01, and CCY cells were compared at various times and positions within the biofilm. The distribution of the CCY was very heterogeneous. It was found in the water channels as well as in regions within biofilm colonies. Many of the yeast cells observed within the biofilm colonies under conditions of low or stopped flow were removed when medium was flowing, suggesting that the yeast was not held in the matrix as tightly as were the bacteria.
利用共焦拉曼显微光谱（CRM）追踪了含胡萝卜素酵母（CCY）在铜绿假单胞菌PA01小菌落变体（SCV）形成的生物膜中的分布情况。通过光谱特征区分了SCV PA01和CCY细胞，并利用C-H弯曲或拉伸信号监测了总生物量的分布。在生物膜内的不同时间和位置比较了总生物量、PA01和CCY细胞的分布。CCY的分布非常不均匀。在水通道以及生物膜菌落内部区域都发现了它。在低流量或停止流动条件下在生物膜菌落中观察到的许多酵母细胞在培养基流动时被去除，这表明酵母在基质中的固定不如细菌紧密。
