基于原位拉曼光谱的微流控“片上实验室”平台，用于非破坏性和连续表征铜绿假单胞菌生物膜。
An in situ Raman spectroscopy-based microfluidic "lab-on-a-chip" platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms.
机构信息
Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
出版信息
Chem Commun (Camb). 2015 May 28;51(43):8966-9. doi: 10.1039/c5cc02744f.
Abstract
Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic "lab-on-a-chip" platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platform and correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.
摘要
铜绿假单胞菌生物膜在微流控“片上实验室”平台中进行培养和表征，该平台采用共焦拉曼显微镜以非破坏性的方式进行。这种无标记平台可定量生物膜的形成，并且与共聚焦激光扫描显微镜具有良好的相关性。这种拉曼微流控平台还可以区分不同发育阶段的生物膜。
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