• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于原位拉曼光谱的微流控“片上实验室”平台，用于非破坏性和连续表征铜绿假单胞菌生物膜。

An in situ Raman spectroscopy-based microfluidic "lab-on-a-chip" platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms.

机构信息

Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.

出版信息

Chem Commun (Camb). 2015 May 28;51(43):8966-9. doi: 10.1039/c5cc02744f.

DOI:10.1039/c5cc02744f
PMID:25929246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4433399/
Abstract

Pseudomonas aeruginosa biofilm was cultivated and characterized in a microfluidic "lab-on-a-chip" platform coupled with confocal Raman microscopy in a non-destructive manner. Biofilm formation could be quantified by this label-free platform and correlated well with confocal laser scanning microscopy. This Raman-microfluidic platform could also discriminate biofilms at different developmental stages.

摘要

铜绿假单胞菌生物膜在微流控“片上实验室”平台中进行培养和表征，该平台采用共焦拉曼显微镜以非破坏性的方式进行。这种无标记平台可定量生物膜的形成，并且与共聚焦激光扫描显微镜具有良好的相关性。这种拉曼微流控平台还可以区分不同发育阶段的生物膜。

相似文献

1
An in situ Raman spectroscopy-based microfluidic "lab-on-a-chip" platform for non-destructive and continuous characterization of Pseudomonas aeruginosa biofilms.基于原位拉曼光谱的微流控“片上实验室”平台，用于非破坏性和连续表征铜绿假单胞菌生物膜。
Chem Commun (Camb). 2015 May 28;51(43):8966-9. doi: 10.1039/c5cc02744f.
2
Spatial organization of Pseudomonas aeruginosa biofilms probed by combined matrix-assisted laser desorption ionization mass spectrometry and confocal Raman microscopy.联合基质辅助激光解吸电离质谱和共聚焦拉曼显微镜对铜绿假单胞菌生物膜的空间组织进行探测
Analyst. 2014 Nov 21;139(22):5700-8. doi: 10.1039/c4an00435c.
3
A Microfluidic Approach to Investigating a Synergistic Effect of Tobramycin and Sodium Dodecyl Sulfate on Pseudomonas aeruginosa Biofilms.一种用于研究妥布霉素和十二烷基硫酸钠对铜绿假单胞菌生物膜协同作用的微流控方法。
Anal Sci. 2016;32(1):67-73. doi: 10.2116/analsci.32.67.
4
Confocal Raman microspectroscopy as a tool for studying the chemical heterogeneities of biofilms in situ.共聚焦拉曼显微光谱法作为原位研究生物膜化学异质性的工具。
J Appl Microbiol. 2007 Nov;103(5):1808-20. doi: 10.1111/j.1365-2672.2007.03413.x.
5
A confocal Raman microscopy study of the distribution of a carotene-containing yeast in a living Pseudomonas aeruginosa biofilm.一项关于含胡萝卜素酵母在活的铜绿假单胞菌生物膜中分布的共聚焦拉曼显微镜研究。
Appl Spectrosc. 2008 Sep;62(9):975-83. doi: 10.1366/000370208785793245.
6
Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering.通过表面增强共振拉曼散射检测和成像铜绿假单胞菌生物膜群落中的群体感应。
Nat Mater. 2016 Nov;15(11):1203-1211. doi: 10.1038/nmat4720. Epub 2016 Aug 8.
7
Electrochemical Surface-Enhanced Raman Spectroscopy of Pyocyanin Secreted by Pseudomonas aeruginosa Communities.电化学表面增强拉曼光谱法研究铜绿假单胞菌群落分泌的绿脓菌素。
Langmuir. 2019 May 28;35(21):7043-7049. doi: 10.1021/acs.langmuir.9b00184. Epub 2019 May 14.
8
Multichannel Microfluidic Platform for Temporal-Spatial Investigation of Niche Roles of Pseudomonas aeruginosa and Escherichia coli within a Dual-Species Biofilm.用于时空研究铜绿假单胞菌和大肠杆菌在双物种生物膜中生态位作用的多通道微流控平台。
Appl Environ Microbiol. 2023 Jul 26;89(7):e0065123. doi: 10.1128/aem.00651-23. Epub 2023 Jun 29.
9
Spatial Mapping of Pyocyanin in Pseudomonas Aeruginosa Bacterial Communities Using Surface Enhanced Raman Scattering.利用表面增强拉曼散射对铜绿假单胞菌细菌群落中的绿脓菌素进行空间映射
Appl Spectrosc. 2017 Feb;71(2):215-223. doi: 10.1177/0003702816654167. Epub 2016 Jul 20.
10
Microfluidic bioanalytical flow cells for biofilm studies: a review.微流控生物分析流动池在生物膜研究中的应用：综述。
Analyst. 2018 Dec 17;144(1):68-86. doi: 10.1039/c8an01526k.

引用本文的文献

1
Alpha-synuclein aggregation induces prominent cellular lipid changes as revealed by Raman spectroscopy and machine learning analysis.拉曼光谱和机器学习分析表明，α-突触核蛋白聚集会引发显著的细胞脂质变化。
Brain Commun. 2025 Apr 3;7(2):fcaf133. doi: 10.1093/braincomms/fcaf133. eCollection 2025.
2
Recent advances in surface enhanced Raman spectroscopy for bacterial pathogen identifications.表面增强拉曼光谱技术在细菌病原体鉴定中的最新进展。
J Adv Res. 2023 Sep;51:91-107. doi: 10.1016/j.jare.2022.11.010. Epub 2022 Dec 19.
3
Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate of .对由环境分离株形成的生物膜的动力学和组成的见解 。 你提供的原文似乎不完整，“of”后面缺少具体内容。
Front Microbiol. 2022 Jul 5;13:877060. doi: 10.3389/fmicb.2022.877060. eCollection 2022.
4
Comparison of Two Biofilm Models.两种生物膜模型的比较
Microorganisms. 2021 Sep 26;9(10):2035. doi: 10.3390/microorganisms9102035.
5
Microfluidic Microbial Bioelectrochemical Systems: An Integrated Investigation Platform for a More Fundamental Understanding of Electroactive Bacterial Biofilms.微流控微生物生物电化学系统：一个用于更深入理解电活性细菌生物膜的综合研究平台。
Microorganisms. 2020 Nov 23;8(11):1841. doi: 10.3390/microorganisms8111841.
6
Testing Anti-Biofilm Polymeric Surfaces: Where to Start?测试抗生物膜聚合物表面：从何处开始？
Int J Mol Sci. 2019 Aug 3;20(15):3794. doi: 10.3390/ijms20153794.
7
In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation.基于生物膜亲水性的原位检测用于环境生物膜形成。
Sci Rep. 2019 May 30;9(1):8070. doi: 10.1038/s41598-019-44167-6.
8
High Correlation Between Structure Development and Chemical Variation During Biofilm Formation by .. 在生物膜形成过程中结构发育与化学变化之间的高度相关性
Front Microbiol. 2018 Aug 14;9:1881. doi: 10.3389/fmicb.2018.01881. eCollection 2018.
9
Environmental Stress-Induced Bacterial Lysis and Extracellular DNA Release Contribute to Campylobacter jejuni Biofilm Formation.环境胁迫诱导的细菌裂解和细胞外 DNA 释放有助于空肠弯曲菌生物膜的形成。
Appl Environ Microbiol. 2018 Feb 14;84(5). doi: 10.1128/AEM.02068-17. Print 2018 Mar 1.
10
Label-free NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids.通过原位合成银胶体实现食源细菌的无标记近红外表面增强拉曼光谱鉴别与检测
J Nanobiotechnology. 2015 Jun 25;13:45. doi: 10.1186/s12951-015-0106-4.

本文引用的文献

1
Persister cells in biofilm associated infections.生物膜相关感染中的持留菌。
Adv Exp Med Biol. 2015;831:1-9. doi: 10.1007/978-3-319-09782-4_1.
2
Broad-spectrum anti-biofilm peptide that targets a cellular stress response.靶向细胞应激反应的广谱抗生物膜肽。
PLoS Pathog. 2014 May 22;10(5):e1004152. doi: 10.1371/journal.ppat.1004152. eCollection 2014 May.
3
Biofilm responses to smooth flow fields and chemical gradients in novel microfluidic flow cells.新型微流控流动细胞中生物膜对平稳流场和化学梯度的响应。
Biotechnol Bioeng. 2014 Mar;111(3):597-607. doi: 10.1002/bit.25107. Epub 2013 Sep 30.
4
Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies.细菌生物膜的形成是一种多细胞适应现象：抗生素耐药性和新的治疗策略。
Curr Opin Microbiol. 2013 Oct;16(5):580-9. doi: 10.1016/j.mib.2013.06.013. Epub 2013 Jul 20.
5
Detection of receptor-induced glycoprotein conformational changes on enveloped virions by using confocal micro-Raman spectroscopy.利用共焦显微拉曼光谱检测包膜病毒上受体内化诱导的糖蛋白构象变化。
J Virol. 2013 Mar;87(6):3130-42. doi: 10.1128/JVI.03220-12. Epub 2013 Jan 2.
6
Evidence for phenotypic plasticity among multihost Campylobacter jejuni and C. coli lineages, obtained using ribosomal multilocus sequence typing and Raman spectroscopy.使用核糖体多位点序列分型和拉曼光谱技术获得的多宿主空肠弯曲菌和大肠弯曲菌谱系中表型可塑性的证据。
Appl Environ Microbiol. 2013 Feb;79(3):965-73. doi: 10.1128/AEM.02521-12. Epub 2012 Nov 30.
7
A microfluidic device for high throughput bacterial biofilm studies.一种用于高通量细菌生物膜研究的微流控装置。
Lab Chip. 2012 Mar 21;12(6):1157-63. doi: 10.1039/c2lc20800h. Epub 2012 Feb 9.
8
Label-free Raman observation of cytochrome c dynamics during apoptosis.无标记拉曼观察细胞色素 c 在细胞凋亡过程中的动力学。
Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):28-32. doi: 10.1073/pnas.1107524108. Epub 2011 Dec 19.
9
Should we stay or should we go: mechanisms and ecological consequences for biofilm dispersal.我们是该留下还是离开：生物膜分散的机制和生态后果。
Nat Rev Microbiol. 2011 Nov 28;10(1):39-50. doi: 10.1038/nrmicro2695.
10
New device for high-throughput viability screening of flow biofilms.高通量流态生物膜活力筛选的新装置。
Appl Environ Microbiol. 2010 Jul;76(13):4136-42. doi: 10.1128/AEM.03065-09. Epub 2010 Apr 30.