Jehlička Jan, Edwards Howell G M, Oren Aharon
Charles University in Prague, Institute of Geochemistry, Mineralogy and Mineral Resources, Prague, Czech Republic.
Appl Environ Microbiol. 2014 Jun;80(11):3286-95. doi: 10.1128/AEM.00699-14. Epub 2014 Mar 28.
Raman spectroscopy is a rapid nondestructive technique providing spectroscopic and structural information on both organic and inorganic molecular compounds. Extensive applications for the method in the characterization of pigments have been found. Due to the high sensitivity of Raman spectroscopy for the detection of chlorophylls, carotenoids, scytonemin, and a range of other pigments found in the microbial world, it is an excellent technique to monitor the presence of such pigments, both in pure cultures and in environmental samples. Miniaturized portable handheld instruments are available; these instruments can be used to detect pigments in microbiological samples of different types and origins under field conditions.
拉曼光谱是一种快速无损技术,可提供有关有机和无机分子化合物的光谱和结构信息。该方法在颜料表征方面有广泛应用。由于拉曼光谱对检测叶绿素、类胡萝卜素、藻青素以及微生物界中发现的一系列其他色素具有高灵敏度,因此它是监测纯培养物和环境样品中此类色素存在情况的出色技术。现已有小型便携式手持仪器;这些仪器可用于在野外条件下检测不同类型和来源的微生物样品中的色素。