Pimenta-Marques Ana, Tostões Rui, Marty Thomas, Barbosa Vítor, Lehmann Ruth, Martinho Rui Gonçalo
Instituto Gulbenkian de Ciência, Rua da Quinta Grande, n 6, 2781-901 Oeiras, Portugal.
Dev Biol. 2008 Nov 15;323(2):197-206. doi: 10.1016/j.ydbio.2008.08.021. Epub 2008 Aug 29.
During mitosis different types of cells can have differential requirements for chromosome segregation. We isolated two new alleles of the separation anxiety gene (san). san was previously described in both Drosophila and in humans to be required for centromeric sister chromatid cohesion (Hou et al., 2007; Williams et al., 2003). Our work confirms and expands the observation that san is required in vivo for normal mitosis of different types of somatic cells. In addition, we suggest that san is also important for the correct resolution of chromosomes, implying a more general function of this acetyltransferase. Surprisingly, during oogenesis we cannot detect mitotic defects in germ line cells mutant for san. We hypothesize the female germ line stem cells have differential requirements for mitotic sister chromatid cohesion.
在有丝分裂过程中，不同类型的细胞对染色体分离可能有不同的要求。我们分离出了分离焦虑基因（san）的两个新等位基因。之前在果蝇和人类中都已描述过，san是着丝粒姐妹染色单体黏连所必需的（侯等人，2007年；威廉姆斯等人，2003年）。我们的研究工作证实并扩展了以下观察结果：san在体内是不同类型体细胞正常有丝分裂所必需的。此外，我们认为san对于染色体的正确分离也很重要，这意味着这种乙酰转移酶具有更广泛的功能。令人惊讶的是，在卵子发生过程中，我们在san突变的生殖系细胞中未检测到有丝分裂缺陷。我们推测雌性生殖系干细胞对有丝分裂姐妹染色单体黏连有不同的要求。
