Wheelock G D, Sieber K P, Hagedorn H H
Department of Entomology, Cornell University, Ithaca, NY 14853.
J Chromatogr. 1991 Apr 12;542(2):508-14. doi: 10.1016/s0021-9673(01)88785-9.
Methods were developed for the isolation of the egg development neurosecretory hormone, EDNH, from heads of the mosquito Aedes aegypti. This hormone stimulates ecdysone production by ovaries. Methods used for the successful isolation of insulin-like peptides from vertebrate tissues were modified to develop a four-step procedure involving extraction in acidified ethanol, precipitation by neutralization, followed by sequential separation on size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography columns.
已开发出从埃及伊蚊头部分离卵子发育神经分泌激素（EDNH）的方法。这种激素可刺激卵巢产生蜕皮激素。对用于从脊椎动物组织中成功分离胰岛素样肽的方法进行了改进，以开发出一种四步程序，包括在酸化乙醇中提取、通过中和沉淀，然后依次在尺寸排阻、离子交换和反相高效液相色谱柱上进行分离。
