通过高效液相色谱法从蚊子头部快速分离出一种神经激素。

Rapid isolation of a neurohormone from mosquito heads by high-performance liquid chromatography.

作者信息

Wheelock G D, Sieber K P, Hagedorn H H

机构信息

Department of Entomology, Cornell University, Ithaca, NY 14853.

出版信息

J Chromatogr. 1991 Apr 12;542(2):508-14. doi: 10.1016/s0021-9673(01)88785-9.

DOI:10.1016/s0021-9673(01)88785-9

Abstract

Methods were developed for the isolation of the egg development neurosecretory hormone, EDNH, from heads of the mosquito Aedes aegypti. This hormone stimulates ecdysone production by ovaries. Methods used for the successful isolation of insulin-like peptides from vertebrate tissues were modified to develop a four-step procedure involving extraction in acidified ethanol, precipitation by neutralization, followed by sequential separation on size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography columns.

摘要

已开发出从埃及伊蚊头部分离卵子发育神经分泌激素（EDNH）的方法。这种激素可刺激卵巢产生蜕皮激素。对用于从脊椎动物组织中成功分离胰岛素样肽的方法进行了改进，以开发出一种四步程序，包括在酸化乙醇中提取、通过中和沉淀，然后依次在尺寸排阻、离子交换和反相高效液相色谱柱上进行分离。

相似文献

1

Rapid isolation of a neurohormone from mosquito heads by high-performance liquid chromatography.通过高效液相色谱法从蚊子头部快速分离出一种神经激素。

J Chromatogr. 1991 Apr 12;542(2):508-14. doi: 10.1016/s0021-9673(01)88785-9.

2

Multiple forms of cerebral peptides with steroidogenic functions in pupal and adult brains of the yellow fever mosquito, Aedes aegypti.在黄热病蚊子埃及伊蚊的蛹脑和成虫脑中具有类固醇生成功能的多种脑肽形式。

Mol Cell Endocrinol. 1987 Mar;50(1-2):3-14. doi: 10.1016/0303-7207(87)90071-2.

3

Partial purification of egg development neurosecretory hormone with reverse-phase liquid chromatographic techniques.用反相液相色谱技术对卵发育神经分泌激素进行部分纯化。

Life Sci. 1983 Nov 7;33(19):1925-31. doi: 10.1016/0024-3205(83)90677-x.

4

Egg maturation and ecdysiotropic activity in extracts of mosquito (Aedes aegypti) heads.埃及伊蚊头部提取物中的卵子成熟和蜕皮激素活性

Gen Comp Endocrinol. 1985 Nov;60(2):196-203. doi: 10.1016/0016-6480(85)90314-4.

5

The influence of the brain hormone on retention of blood in the mid-gut of the mosquito Aedes aegypti (L.). III. The involvement of the ovaries and ecdysone.脑激素对埃及伊蚊（L.）中肠血液潴留的影响。III. 卵巢和蜕皮激素的作用。

Proc R Soc Lond B Biol Sci. 1979 Aug 31;205(1160):411-22. doi: 10.1098/rspb.1979.0074.

6

An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti.一种胰岛素样肽调节埃及伊蚊的卵子成熟和新陈代谢。

Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5716-21. doi: 10.1073/pnas.0800478105. Epub 2008 Apr 7.

7

Sequencing and characterization of trypsin modulating oostatic factor (TMOF) from the ovaries of the grey fleshfly, Neobellieria (Sarcophaga) bullata.从灰肉蝇（新贝氏丽蝇，即麻蝇属）卵巢中对胰蛋白酶调节卵发育抑制因子（TMOF）进行测序和特性分析。

Regul Pept. 1994 Feb 3;50(1):61-72. doi: 10.1016/0167-0115(94)90192-9.

8

Isolation and primary structure of a neuropeptide hormone from Heliothis zea with hypertrehalosemic and adipokinetic activities.从具有高海藻糖血症和脂肪动员活性的烟芽夜蛾中分离出一种神经肽激素及其一级结构。

Biochem Biophys Res Commun. 1988 Aug 30;155(1):344-50. doi: 10.1016/s0006-291x(88)81091-x.

9

Ecdysone conjugates: isolation and identification.蜕皮激素缀合物：分离与鉴定

Methods Enzymol. 1985;111:411-9. doi: 10.1016/s0076-6879(85)11025-6.

10

Control of ovarian steroidogenesis in insects: a locust neurohormone is active in vitro on blowfly ovaries.昆虫卵巢甾体生成的调控：一种蝗虫神经激素在体外对家蝇卵巢有活性。

Gen Comp Endocrinol. 2009 Sep 15;163(3):292-7. doi: 10.1016/j.ygcen.2009.04.034. Epub 2009 May 20.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。